Analysis of antibiotic residues in milk from healthy dairy cows treated with bovine mastitis ointment using ultra-performance liquid chromatography coupled with electrospray tandem mass spectrometry.
10.14405/kjvr.2016.56.4.233
- Author:
Eun Kee PARK
1
;
Yong Jae RYU
;
Chun Nam CHA
;
Chang Yeul YOO
;
Suk KIM
;
Hu Jang LEE
Author Information
1. Medical Humanities and Social Medicine, College of Medicine, Kosin University, Busan 46241, Korea.
- Publication Type:Original Article
- Keywords:
UPLC-MS/MS;
bovine milk;
neomycin;
penicillin G;
streptomycin
- From:Korean Journal of Veterinary Research
2016;56(4):233-239
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was conducted to analyze penicillin G (PEG), streptomycin (STR) and neomycin (NEO) residues in milk of healthy lactating cows. Milk samples were collected from all four quarters of 12 dairy cows 2–7 days after intramammary infusions of an ointment containing PEG, STR and NEO once (n = 4; group I) or twice (n = 4, group II) daily. Ultra-performance liquid chromatography coupled with electrospray tandem mass spectrometry was used to determine the antibiotic residues in the samples. The correlation coefficient (r 2) of the calibration curves for all antibiotics was > 0.999 and the limits of detection and quantification were 0.002–0.005 µg/mL and 0.007–0.02 µg/mL, respectively. Recovery rates were ranged from 75.5 to 92.3%. In group I, PEG, STR and NEO residues were detected in milk at 2, 3 and 2 days post-treatment, respectively, which were below the maximum residue limit (MRL). In group II, PEG, STR and NEO residues were detected in milk at 2, 3 and 3 days post-treatment, respectively, which were bellow the MRL. These results suggest that a 3-day for milk withdrawal period after the ointment treatment might be sufficient for reduction of the antibiotic residues below the MRL.
