Immunohistochemical localization of eight phospholipase C isozymes in pancreatic islets of the mouse.
- Author:
Sung Sook KIM
1
;
Ki Sun JUN
;
Myung Jin JEONG
;
Sung Ho RYU
;
Pann Ghill SUH
;
Hee Sup SHIN
Author Information
1. National Creative Research Initiative Center for Calcium and Learning, Pohang, Korea.
- Publication Type:Original Article
- Keywords:
pancreatic islet;
beta cell;
insulin;
isozymes;
phospholipase C
- MeSH:
Animal;
Antibodies, Monoclonal;
Glucagon/analysis;
Insulin/analysis;
Islets of Langerhans/cytology/*enzymology;
Isoenzymes/analysis/immunology;
Mice;
Mice, Inbred C57BL;
Mice, Knockout;
Phospholipase C/*analysis/immunology
- From:Experimental & Molecular Medicine
2001;33(3):164-168
- CountryRepublic of Korea
- Language:English
-
Abstract:
The possible involvement of phospholipase C (PLC) in the regulation of insulin secretion is not clearly understood and neither its isozymes expressed nor cellular localization in the pancreatic islets is known. By using specific monoclonal antibodies, we have investigated the expression and localization of eight different PLC isozymes, beta1, beta2, beta3, beta4, gamma1, gamma2, delta1, and delta2, in the pancreatic islets of adult mice. Immunohistochemical analysis carried out on paraffin embedded sections showed a distinct pattern of expression for each of the PLC isozymes. In the central part of the islets containing beta cells, a high level of beta4 and moderate levels of beta3 and gamma1 were expressed, whereas PLC-beta1 and -gamma1 were abundantly expressed in the exocrine pancreas. These results demonstrated the heterogeneity in expression of the phospholipase C isozymes in pancreatic islets. It is conceivable that these isozymes are coupled to different receptors and perform selective tasks in the regulation of insulin secretion for glucose homeostasis.
