Comparison of biocompatibility of four root perforation repair materials.
10.5395/JKACD.2009.34.3.192
- Author:
Min Kyung KANG
1
;
In Ho BAE
;
Jeong Tae KOH
;
Yun Chan HWANG
;
In Nam HWANG
;
Won Mann OH
Author Information
1. Department of Conservative Dentistry, School of Dentistry, Chonnam National University, Korea. wmoh@chonnam.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Dycal;
Glass-ionomer cement;
MTA;
Portland cement;
Biocompatibility;
Cytotoxicity
- MeSH:
Acrylic Resins;
Aluminum Compounds;
Calcium Compounds;
Calcium Hydroxide;
Drug Combinations;
Glass Ionomer Cements;
Glutamates;
Guanine;
Humans;
Hydroxides;
Microscopy, Electron, Scanning;
Minerals;
Oxides;
Silicates;
Silicon Dioxide;
Pemetrexed
- From:Journal of Korean Academy of Conservative Dentistry
2009;34(3):192-198
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was carried out in order to determine in vitro biocompatibility of white mineral trioxide aggregate (MTA), and to compare it with that of the commonly used materials, i. e. calcium hydroxide liner (Dycal), glass ionomer cement (GIC), and Portland cement which has a similar composition of MTA. To assess the biocompatibility of each material, cytotoxicity was examined using MG-63 cells. The degree of cytotoxicity was evaluated by scanning electron microscopy (SEM) and a colorimetric method, based on reduction of the tetrazolium salt 2,3 bis {2methoxy 4nitro 5[(sulfenylamino) carbonyl] 2H tetrazolium hydroxide} (XTT) assay. The results of SEM revealed the cells in contact with GIC, MTA, and Portland cement at 1 and 3 days were apparently healthy. In contrast, cells in the presence of Dycal appeared rounded and detached. In XTT assay, the cellular activities of the cells incubated with all the test materials except Dycal were similar, which corresponded with the SEM observation. The present study supports the view that MTA is a very biocompatible root perforation repair material. It also suggests that cellular response of Portland cement and GIC are very similar to that of MTA.
