Sequence comparisons of 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I of Metagonimus yokogawai, M. takahashii and M. miyatai.
10.3347/kjp.2004.42.3.129
- Author:
Soo Ung LEE
;
Sun HUH
;
Woon Mok SOHN
;
Jong Yil CHAI
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords:
Metagonimus;
Heterophyidae;
polymerase chain reaction;
sequence analysis;
DNA;
classification
- MeSH:
Animals;
Base Sequence;
Comparative Study;
DNA, Helminth/*chemistry/genetics;
DNA, Mitochondrial/chemistry/genetics;
DNA, Ribosomal/chemistry/genetics;
Electron Transport Complex IV/chemistry/*genetics;
Heterophyidae/classification/enzymology/*genetics;
Molecular Sequence Data;
Phylogeny;
RNA, Ribosomal, 28S/chemistry/*genetics;
Research Support, Non-U.S. Gov't;
Sequence Alignment;
Trematode Infections/*parasitology
- From:The Korean Journal of Parasitology
2004;42(3):129-135
- CountryRepublic of Korea
- Language:English
-
Abstract:
We compared the DNA sequences of the genus Metagonimus: M. yokogawai, M. takahashii, and M. miyatai. We obtained 28S D1 ribosomal DNA (rDNA) and mitochondrial cytochrome c oxidase subunit I (mtCOI) fragments from the adult worms by PCR, that were cloned and sequenced. Phylogenetic relationships inferred from the nucleotide sequences of the 28S D1 rDNA and mtCOI gene. M. takahashii and M. yokogawai are placed in the same clade supported by DNA sequence and phylogenic tree analysis in 28S D1 rDNA and mtCOI gene region. The above findings tell us that M. takahashii is closer to M. yokogawai than to M. miyatai genetically. This phylogenetic data also support the nomination of M. miyatai as a separate species.
