Expressed sequence tags analysis of Blattella germanica.
10.3347/kjp.2005.43.4.149
- Author:
Hyang Suk CHUNG
;
Tai Hyun YU
;
Bong Jin KIM
;
Sun Mi KIM
;
Joo Yeong KIM
;
Hak Sun YU
;
Hae Jin JEONG
;
Mee Sun OCK
- Publication Type:Original Article
- Keywords:
Blattella germanica;
cDNA library;
ESTs;
BLAST search;
novel genes
- MeSH:
Sequence Alignment;
Reverse Transcriptase Polymerase Chain Reaction;
Molecular Sequence Data;
Male;
Female;
*Expressed Sequence Tags;
Blattellidae/*genetics;
Base Sequence;
Animals
- From:The Korean Journal of Parasitology
2005;43(4):149-156
- CountryRepublic of Korea
- Language:English
-
Abstract:
Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About 42% (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling pathways, and proteins of unknown function. Eighty clones were not well-matched by database searches, and these represent new B. germanica-specific ESTs. Some genes which drew our attention are discussed. The information obtained increases our understanding of the B. germanica genome.
