Effects of Mycobacterium leprae , M . leprae Phenolic Glycolipid - 1 and Cytokines on the Nitric Oxide Generation of RAW 264 . 7 Macrophages.
- Author:
Seok Don PARK
;
Jae Sung LEE
;
Bok Soo LEE
;
Chang Duk JUN
;
Hun Taeg CHUNG
;
Jong Gu KIM
- Publication Type:Original Article
- Keywords:
M. leprae;
PGL-L;
Macrophage;
IFN-y;
TNF-a;
Nitric Oxidi
- MeSH:
Cell Line;
Cytokines*;
Leprosy;
Macrophages*;
Mycobacterium leprae*;
Mycobacterium*;
Necrosis;
Nitric Oxide*;
Phenol*
- From:Korean Journal of Dermatology
1994;32(2):234-244
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Background: Nitric oxide(NO) has been reproted to play an important role in macrophage-mediated microbicidal capacity for a variety of intracellular pathogens. NO generation is used as an indicator of microbicidal function of macrophages. OBJECTIVE: Our purpose is to investigate the production of NO rom macrophages phagocytized with Mycobacterium leprae or M. leprae phenolic glycolipid-1(PGL-1) for the purpose of elucidating the pathogenesis of leprosy. METHODS: We used a murine macrophage cell line, RAW 264.7. Macrophages were incubated with dead M. leprae or PGL-1, respectively and then treated with interfer n-gamma(IFN-r) and/or tumor necrosis factor-alpha(TNF-a). The release of NO was determined spectrophotometrically by measuring nitrite. RESULTS: M. Leprae and PGL-1 failed to stimulnte NO secretion execept at high bacteria-to-cell rations(50:1)and at the higheat concentrat,ion(100pg/ml) of PGL-1. IFN-r or IFN-r plus TNF-a markedly stimulated macrophages phagocyt,ized with M. leprae or PGL-1 to release NO . CONCLUSION: Defective IFN-r-dependent NO production of macrophages may be an important factor in the pathogenesis of leprosy.
