Comparison of BACTEC Plus Aerobic/F Media and BacT/Alert FA Media to Detect Bacteria in Blood Culture Bottles Containing Peak Therapeutic Levels of Antimicrobials.
10.5145/KJCM.2010.13.4.151
- Author:
Jin Young LEE
1
;
Jong Hee HONG
;
Miae LEE
Author Information
1. Department of Laboratory Medicine, Ewha Womans University School of Medicine, Seoul, Korea. miae@ewha.ac.kr
- Publication Type:Original Article
- Keywords:
Blood culture;
Antimicrobial removal system;
BACTEC Plus Aerobic/F;
BacT/Alert FA
- MeSH:
Ampicillin;
Anti-Bacterial Agents;
Bacteria;
Cefotaxime;
Cefoxitin;
Ceftriaxone;
Cephalosporins;
Ciprofloxacin;
Gram-Negative Bacteria;
Gram-Positive Bacteria;
Humans;
Methicillin Resistance;
Oxacillin;
Pneumonia;
Vancomycin
- From:Korean Journal of Clinical Microbiology
2010;13(4):151-156
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Blood culture bottles with an antimicrobial removal system have been developed for patients treated with antibiotics. This study compared the ability of BACTEC Plus Aerobic/F bottles (Becton Dickinson, USA, BACTEC Plus) and BacT/Alert FA bottles (bio-Merieux Vitek, France) to effectively remove antimicrobials. METHODS: BACTEC Plus and BacT/Alert FA bottles were spiked with 5 mL human blood, peak therapeutic concentrations of 9 antimicrobials and 7 type strains. Three rounds of duplicate testing were completed per antimicrobial/strain combination and growth control without antimicrobials. The time to detection (TTD) and recovery rates for bacteria were compared for both systems. RESULTS: Overall, the BACTEC Plus and BacT/Alert FA recovered 76% (128/168) and 34% (57/168) of strains from test bottles, respectively. BACTEC Plus detected all of gram-positive bacteria except S. pneumoniae with ampicillin and ceftriaxone, but BacT/Alert FA detected 0~50% of gram-positive bacteria except E. faecalis with vancomycin and methicillin-resistant S. aureus with oxacillin. In presence of cefepime, cefotaxime, cefoxitin and ceftriaxone, BACTEC Plus detected 33~100% of gram-negative bacteria, but BacT/ Alert FA did not detect gram-negative bacteria at all. In presence of ciprofloxacin, BacT/Alert FA detected 100% of E. coli and K. pneumoniae compared with 33% of those for BACTEC Plus. Overall, TTD of BACTEC Plus was shorter than that of BacT/Alert FA except in detecting gram-negative bacteria with ciprofloxacin (P<0.05). CONCLUSION: BACTEC Plus Aerobic/F media containing peak therapeutic levels of antimicrobials are more effective and faster detection of bacteria than BacT/Alert FA media.
