Performance of the VITEK2 System for Detection of Inducible Clindamycin Resistance in Staphylococci.
10.5145/KJCM.2010.13.4.157
- Author:
Mi Kyung KIM
1
;
Jong Hee HONG
;
Miae LEE
Author Information
1. Department of Laboratory Medicine, Ewha Womans University School of Medicine, Seoul, Korea. miae@ewha.ac.kr
- Publication Type:Original Article
- Keywords:
Inducible clindamycin resistance;
Staphylococcus aureus;
VITEK2
- MeSH:
Clindamycin;
Erythromycin;
Sensitivity and Specificity;
Staphylococcus;
Staphylococcus aureus
- From:Korean Journal of Clinical Microbiology
2010;13(4):157-161
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The Clinical and Laboratory Standards Institute (CLSI) recommends testing for inducible clindamycin resistance in clindamycin non-resistant and erythromycin resistant (CNR-ER) staphylococci by using a D-zone test. Recently, the VITEK2 system was developed to detect inducible clindamycin resistance in staphylococci. We evaluated the performance of the VITEK2 system by comparing it with a D-zone test. METHODS: In detecting inducible clindamycin resistance, a total of 142 clinical isolates of staphylococci were tested by using the VITEK2 Antimicrobial Susceptibility Test (AST)-P601 card (bioMerieux, Marcy l'Etoile, France) and the D-zone test. Of the 142 isolates of staphylococci tested, 114 were CNR-ER staphylococci [40 coagulase-negative staphylococci (CoNS), 74 Staphylococcus aureus] and 28 were staphylococci, either resistant or susceptible to clindamycin and erythromycin (1 CoNS and 27 S. aureus). RESULTS: Of the 114 CNR-ER staphylococci, 98.6% (73/74) of S. aureus and 32.5% (13/40) of CoNS were inducible clindamycin resistant according to the Dzone test. Overall sensitivity and specificity of the VITEK2 system were 98.8% (85/86) and 98.2% (55/56) respectively, and the agreement between the VITEK2 system and the D-zone test was 98.6% (140/142). CONCLUSION: The VITEK2 system shows high concordance with a D-zone test. The inducible clindamycin resistance in staphylococci can be detected easily and conveniently by the VITEK2 system.
