The Change of Interleukin-1beta & Nitric Oxide in Tear after Excimer Laser Photorefractive Keratectomy.
- Author:
Ji Han RYOU
1
;
Noh Young BAE
;
Kyung Hwan SHYN
;
Jae Chan KIM
Author Information
1. Department of Opthalmology, College of Medicine, Chung-ang University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Excimer laser PRK;
Interleukin-1beta;
Nitric oxide;
Tear
- MeSH:
Animals;
Apoptosis;
Cornea;
Corneal Opacity;
Interleukin-1beta*;
Lasers, Excimer*;
Nitric Oxide*;
Photorefractive Keratectomy*;
Rats;
Tears*
- From:Journal of the Korean Ophthalmological Society
1998;39(1):25-36
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was performed to investigate the amount of IL-1beta & NO in tear film after Excimer laser PRK and to determine whether NO induces cytotoxicity associated with apoptosis of keratocytes. Twelve myopes underwent PRK and were divided into 3 groups according to spherical equivalent. IL-1beta and NO production were measured at preop and postop 1, 4, 7 days, and expression of NOS and apoptosis of keratocytes were evaluated in the corneas of rats at 2 days after PRK. IL-1beta and NO production were significantly increased at postop 1 day(p<0.01) and then gradually decreased to preop levels at postop 7 days. The corneas of rats at 2 days after PTK revealed increased expression of NOS(iNOS, bNOS) and increased apoptosis of keratocytes in stroma of cornea. The degree of corneal opacity and NO production at postop 1 days were increased, positively correlated to ablation depth. It is suggested that NO production induced by IL-1beta may be related with apoptosis of keratocyte, which can influence on the corneal opacity after PRK.
