The Effect of Hydrophilic Treatment of PMMA Material Using Hydrolysis on Cell Attachment in Vivo (II).
- Author:
Ji Young PARK
1
;
Choun Ki JOO
;
Su Won SEO
;
Jin Hak LEE
Author Information
1. Department of Ophthalmology, Catholic University Medical College Catholic Research.
- Publication Type:In Vitro ; Original Article
- Keywords:
After-Cataract;
Cataract;
Cell attachment;
Hydrophilic PMMA;
PMMA
- MeSH:
Animals;
Cataract;
Cytoplasm;
Diabetes Mellitus;
Endothelium, Corneal;
Hydrolysis*;
Membranes;
Polymethyl Methacrylate*;
Rats;
Uveitis
- From:Journal of the Korean Ophthalmological Society
1998;39(1):73-79
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PMMA(polymethyl Methacrylate) introcular lens(IOL) has been designed for making surface hydrophobic. Hydrophilic IOL has advantages such as less damage of corneal endothelium and less attachment of inflammatory cells on the IOL surface especially in case of uveitis or diabetes mellitus. Therefore, hydrophilic IOL is regarded as a biocompatible IOL. In this study, we made PMMAs hydrophilic using hydrolysis (described in The Effect of Hydrophilic Treatment of PMMA using Hydrolysis on Light Transmission and Cell Attachment in Vitro (I)). The number of PMMA treated in each condition was twelve and each PMMA has been implanted into rat peritoneal space. These PMMAs were examined with inverted light microscope and scanning electron microscope 3 days and 7-14 days after implantation. The number of cell attachment on pMMA-1 or PMMA-2 was about 1/5 of that on PMMA-0 and the difference was statistically significant (p<0.05). There was no statistically significant difference of cell attachment between PMMA-1 and PMMA-2. The cells on PMMA-0 showed prominent cytoplasmic processes, while those on PMMA-1 or PMMA-2 showed little processes. The majority of the surface PMMA-0 was covered with membrane light sheet. Therefore, it was found that treatment of PMMA with Isopropranol and NaOH was useful in making PMMA dydrophilic and decreasing the cell attachment on PMMA in vivo.
