Expression of vascular endothelial growth factor in oral squamous cell carcinoma.
10.5125/jkaoms.2015.41.1.11
- Author:
Seok Kon KIM
1
;
Seung Goo PARK
;
Kyung Wook KIM
Author Information
1. Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University, Cheonan, Korea. kkwoms@dku.edu
- Publication Type:Original Article
- Keywords:
Squamous cell carcinoma;
Vascular endothelial growth factor;
Real-time polymerase chain reaction;
Immunohistochemistry
- MeSH:
Carcinoma in Situ;
Carcinoma, Squamous Cell*;
Humans;
Immunohistochemistry;
Real-Time Polymerase Chain Reaction;
RNA, Messenger;
Up-Regulation;
Vascular Endothelial Growth Factor A*
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2015;41(1):11-18
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: The goal of this study was to determine the correlation of clinicopathological factors and the up-regulation of vascular endothelial growth factor (VEGF) expression in oral squamous cell carcinoma. MATERIALS AND METHODS: Immunohistochemical staining of VEGF and quantitative real-time polymerase chain reaction (RT-PCR) of VEGF mRNA were performed in 20 specimens from 20 patients with oral squamous cell carcinoma and another 20 specimens from 20 patients with carcinoma in situ as a controlled group. RESULTS: The results were as follows: 1) In immunohistochemical study of poorly differentiated and invasive oral squamous cell carcinoma, high-level staining of VEGF was observed. Significant correlation was observed between immunohistochemical VEGF expression and histologic differentiation, tumor size of specimens (Pearson correlation analysis, significance r>0.6, P<0.05). 2) In VEGF quantitative RT-PCR analysis, progressive cancer showed more VEGF expression than carcinoma in situ. Paired-samples analysis determined the difference of VEGF mRNA expression level between cancer tissue and carcinoma in situ tissue, between T1 and T2-4 (Student's t-test, P<0.05). CONCLUSION: These findings suggest that up-regulation of VEGF may play a role in the angiogenesis and progression of oral squamous cell carcinoma.
