Human Muscle Derived Stem Cell Differentiate into Neurons.
- Author:
Young Yul KIM
1
;
Yun Kyung CHO
;
Soon Yong KWON
;
Bo Kun GI
;
Seok Whan SONG
;
Hyung Jun KIM
;
Chang Whan HAN
;
Gil son KHANG
Author Information
1. Department of Orthopedic surgery, Daejeon St. Mary's hospital, College of Medicine, the Catholic University of Korea, Seoul, Korea. hancw0523@hotmail.com
- Publication Type:In Vitro ; Original Article
- Keywords:
Muscle-derived cell;
Neuronal differentiation;
NSE;
NF;
TrkA
- MeSH:
Anterior Cruciate Ligament Reconstruction;
Culture Media;
Desmin;
Flow Cytometry;
Humans*;
Immunohistochemistry;
Muscles;
Neurons*;
Phosphopyruvate Hydratase;
Stem Cells*
- From:Journal of Korean Orthopaedic Research Society
2005;8(1):41-49
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To evaluate the possibility that human muscle derived stem cells (hMDSCs) can be differentiated into neurons in vitro. MATERIAL AND METHODS: Muscle derived stem cells were isolated from the hamstring muscles during the anterior cruciate ligament reconstruction by preplate technique. For the characterization of these cells, desmin staining, CD 34, Sca-1, CD 29 using the Flow cytometry were performed. In the experimental group, neuronal induction media was added to differentiate hMDSCs to neuronal cells. These cells were evaluated by neuronal markers such as neuron-specific enolase (NSE), neurofilament (NF), TrkA using immunocytochemistry. For the control group, no induction media was added. Statstical analyses were performed by use of Kruskal-Wallis H test and Student-Newman-Keuls test (P<0.01). RESULT: Desmin staining was positive in 92.3+/-6%. Flow cytometry was negative for CD 34 and Sca-1. However it was positive for CD 29. (69.4+/-10%). The immunocytochemical result revealed NSE, NF and TrkA positive with 63.2+/-2.3%, 59.2+/-2.5%, 55+/-2.4% respectively. However, these were negative in the control group. CONCLUSION: Our observations indicate that hMDSCs have the capacity to differentiate into neurons in a specialized culture media.
