Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells in PGA Polymeric Scaffolds.
- Author:
Young Yul KIM
1
;
Soon Yong KWON
;
Weon Yoo KIM
;
Dong Youb LEE
;
Soon Hee KIM
;
Chang Whan HAN
;
Gil Son KHANG
Author Information
1. Department of Orthopedic surgery, Daejeon St. Mary's hospital, College of Medicine, the Catholic University of Korea, Seoul, Korea. hancw0523@hotmail.com
- Publication Type:Original Article
- Keywords:
Mesenchymal stem cell;
Chondrogenesis;
Porous polymeric scaffold
- MeSH:
Antibodies;
Cell Culture Techniques;
Chondrogenesis;
Collagen Type II;
Flow Cytometry;
Humans*;
Mesenchymal Stromal Cells*;
Polymers*;
Proteoglycans
- From:Journal of Korean Orthopaedic Research Society
2005;8(1):76-85
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To characterize the chondrogenic potential of human mesenchymal stem cells (MSCs) in porous polymeric scaffolds by poly (glycolic acid) (PGA) as three-dimensional constructs to facilitate chondrogenic differentiation. METHODS: Human MSCs were isolated by percoll gradient method and adherent cell cultures were obtained. Isolated MSCs were characterized with CD 34 and Sca-1 antibodies using flow cytometry. MSCs were seeded in the PGA polymeric scaffolds for 28 days in a specialized defined medium. The control group was examined without the specialized defined medium. The chondrogenesis of MSCs-seeded polymer scaffolds culture was assessed by histology, RT-PCR and 35S-sulfate incorporation. RESULTS: Flow cytometry result showed that CD 34 was negative and Sca-1 was 93+/-10% positive. By the histological analysis from Safranin-O staining, it was confirmed that the chondrogenic differentiated human MSCs expressed chondrocyte-like morphologies. We also observed that type II collagen was expressed by RT-PCR. The degree of proteoglycan synthesis was higher in the experimental group than the control group. CONCLUSION: We have demonstrated that the biodegradable porous polymeric scaffolds and the specialized defined medium is able to provide three-dimensional constructs for inducing chondrogenic differentiation of human MSCs.
