Allium Sativum-mediated Therapy for Experimental Prostate Cancers with In Vivo Tumor Transduction: Effects on Tumor Growth and Development.
- Author:
Won Joon BHANG
;
Jun CHEON
;
Soon Chan KIM
- Publication Type:Original Article
- Keywords:
Allium sativum;
Prostate cancer;
In vivo
- MeSH:
Allium*;
Animals;
Apoptosis;
Carcinogenesis;
Chemoprevention;
Garlic;
Growth and Development*;
Humans;
Incidence;
Mice;
Mice, Nude;
Prostate*;
Prostatic Neoplasms*
- From:Korean Journal of Urology
2001;42(6):663-667
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Allium sativum (AS) has been known to have widespread benefits in reducing some human cancer risk by immune stimualtion and anticarcinogenic activity. In the present study, we evaluated the preventive and antitumor properties of AS as an effective anticancer modifier for human prostate cancer in vivo. MATERIALS AND METHODS: Subcutaneous prostate cancers were established in athymic nude mice with 5x10(5) PC-3 human androgen-indenpendent prostate cancer cells. AS was injected at the site of tumor transplantation on day 1 and one week intervals up to 5 weeks (Experiment I), and into the established tumors sized by 50-60mm(3) weekly for 5 weeks (Experiment II). Therapeutic responses and efficacies of AS for prostate cancers in vivo were determined in separate controlled experiments, and definite histopathological studies were also performed. RESULTS: In vivo studies indicated statistically significant reduction in the incidence of tumor formation with programmed and continuous AS intralesional treatment. For established prostate cancer, AS treatment also demonstrated an inhibitory effect of tumor growth compared with control. Histomorphological and immunohistochemical studies demonstrated marked apoptosis after 5 weeks-AS continuous treatment in Experiment II. CONCLUSIONS: AS had a definite antitumor activity to inhibit tumorigenesis and may modulate tumor growth of prostate cancer in vivo. It is non-toxic, readily avaliable and inexpensive. AS, in the future, may be developed as a novel and effective treatment in chemoprevention for human prostate cancer.
