The evaluation of periodontal ligament cells of rat teeth after low-temperature preservation under high pressure.
10.5395/JKACD.2010.35.4.285
- Author:
Jin Ho CHUNG
1
;
Jin KIM
;
Seong Ho CHOI
;
Eui Seong KIM
;
Jiyong PARK
;
Seung Jong LEE
Author Information
1. Department of Conservative Dentistry, College of Dentistry, Yonsei University, Seoul, Korea. sjlee@yuhs.ac
- Publication Type:Original Article
- Keywords:
Periodontal ligament cell;
MTT;
WST-1;
Viability;
Low temperature preservation under pressure;
Rapid freezing;
Cryopreservation
- MeSH:
Anesthesia;
Animals;
Baths;
Cryopreservation;
Dimethyl Sulfoxide;
Eosine Yellowish-(YS);
Female;
Freezing;
Humans;
Molar;
Nitrogen;
Periodontal Ligament;
Rats;
Tiletamine;
Tooth
- From:Journal of Korean Academy of Conservative Dentistry
2010;35(4):285-294
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The purpose of this study was to evaluate the viability of periodontal ligament cells of rat teeth after low-temperature preservation under high pressure by means of MTT assay, WST-1 assay. 12 teeth of Sprague-Dawley white female rats of 4 week-old were used for each group. Both side of the first and second maxillary molars were extracted as atraumatically as possible under tiletamine anesthesia. The experimental groups were group 1 (Immediate extraction), group 2 (Slow freezing under pressure of 3 MPa), group 3 (Slow freezing under pressure of 2 MPa), group 4 (Slow freezing under no additional pressure), group 5 (Rapid freezing in liquid nitrogen under pressure of 2 MPa), group 6 (Rapid freezing in liquid nitrogen under no additional pressure), group 7 (low-temperature preservation at 0degrees C under pressure of 2 MPa), group 8 (low-temperature preservation at 0degrees C under no additional pressure), group 9 (low-temperature preservation at -5degrees C under pressure of 90 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in 37degrees C water bath, then MTT assay, WST-1 assay were processed. One way ANOVA and Tukey HSD method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization. In both MTT and WST-1 assay, group 7 (0degrees C/2 MPa) showed higher viability of periodontal ligament cells than other group (2-6, 8) and this was statistically significant (p < 0.05), but showed lower viability than group 1, immediate extraction group (no statistical significance). By the results of this study, low-temperature preservation at 0degrees C under pressure of 2 MPa suggest the possibility for long term preservation of teeth.
