High Frequency of Microsatellite Instability in Intestinal-type Gastric Cancer in Korean Patients.
10.3904/kjim.2005.20.2.116
- Author:
Won Hyuk CHOE
1
;
Sun Young LEE
;
Jun Haeng LEE
;
Sang Goon SHIM
;
Young Ho KIM
;
Poong Lyul RHEE
;
Jong Chul RHEE
;
Chang Seok KI
;
Jong Won KIM
;
Sang Yong SONG
;
Jae J KIM
Author Information
1. Department of Internal Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. jjkim@smc.samsung.co.kr
- Publication Type:Original Article ; Comparative Study
- Keywords:
Gastric cancer;
Histology;
Microsatellite instability
- MeSH:
Adenocarcinoma/epidemiology/*genetics/pathology;
Aged;
Base Pair Mismatch/*genetics;
Comparative Study;
Female;
Gene Expression Regulation, Neoplastic;
Genotype;
Humans;
Incidence;
Korea/epidemiology;
Male;
Microsatellite Repeats/*genetics;
Neoplasm Proteins/genetics;
Nuclear Proteins/genetics;
Polymerase Chain Reaction;
RNA, Messenger/genetics;
Retrospective Studies;
Stomach Neoplasms/epidemiology/*genetics/pathology
- From:The Korean Journal of Internal Medicine
2005;20(2):116-122
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Although there have been some reports on microsatellite alterations in gastric cancer, findings are inconsistent regarding the associations between histological classification and microsatellite instability (MSI). In the present study, we attempted to determine whether Lauren's histological subtypes are related with MSI status. METHODS: Paraffin-embedded tissue samples from 14 diffuse-type and 14 intestinal-type gastric adenocarcinomas were matched up according to patient gender and age. Mononucleotide markers (BAT25 and BAT26) and dinucleotide markers (D2S123, D5S346, and D17S250) were used for MSI analyses. Microsatellite genotypes were categorized in terms of high MSI incidence (MSI-H, > 30% positive marker) or low MSI incidence (MSI-L, < 30% positive marker). Losses of hMLH1 and hMSH2 protein expression were immunohistochemically studied. RESULTS: MSI-H was observed in 11 cases (78%) of the 14 intestinal-type cases as compared to 3 (21%) of the 14 diffuse-type cases (p=0.007). In MSI-H tumors, 10 cases (71%) showed losses of hMLH1 protein expression, while 2 cases (14%) in MSI-L tumors showed losses of hMLH1 protein expression (p=0.006). CONCLUSION: MSI-H tumors are more frequently found in intestinal-type gastric cancer, which suggests the possibility that there are different pathogenic pathways in gastric carcinogenesis according to histologic type.
