Involvement of Caenohabditis elegans MAPK Signaling Pathways in Oxidative Stress Response Induced by Silver Nanoparticles Exposure.
- Author:
Ji Yeon ROH
1
;
Hyun Jeong EOM
;
Jinhee CHOI
Author Information
1. School of Environmental Engineering, College of Urban Science, University of Seoul, Seoul 130-743, Korea. jinhchoi@uos.ac.kr
- Publication Type:Original Article
- Keywords:
Caenorhabditis elegans;
Silver nanoparticles;
Mitogen activated protein kinase;
Oxidative stress
- MeSH:
Caenorhabditis elegans;
Gene Expression;
Nanoparticles;
Oxidative Stress;
p38 Mitogen-Activated Protein Kinases;
Protein Kinases;
Reactive Oxygen Species;
Silver
- From:Toxicological Research
2012;28(1):19-24
- CountryRepublic of Korea
- Language:English
-
Abstract:
In the present study, toxicity of silver nanoparticles (AgNPs) was investigated in the nematode, Caenohabditis elegans focusing on the upstream signaling pathway responsible for regulating oxidative stress, such as mitogen-activated protein kinase (MAPK) cascades. Formation of reactive oxygen species (ROS) was observed in AgNPs exposed C. elegans, suggesting oxidative stress as an important mechanism in the toxicity of AgNPs towards C. elegans. Expression of genes in MAPK signaling pathways increased by AgNPs exposure in less than 2-fold compared to the control in wildtype C. elegans, however, those were increased dramatically in sod-3 (gk235) mutant after 48 h exposure of AgNPs (i.e. 4-fold for jnk-1 and mpk-2; 6-fold for nsy-1, sek-1, and pmk-1, and 10-fold for jkk-1). These results on the expression of oxidative stress response genes suggest that sod-3 gene expression appears to be dependent on p38 MAPK activation. The high expressions of the pmk-1 gene 48 h exposure to AgNPs in the sod-3 (gk235) mutant can also be interpreted as compensatory mechanisms in the absence of important stress response genes. Overall results suggest that MAPK-based integrated stress signaling network seems to be involved in defense to AgNPs exposure in C.elegans.
