B-cell-activating factor is a regulator of adipokines and a possible mediator between adipocytes and macrophages.
- Author:
Mi Young KIM
1
;
Do Hwan KIM
;
Myoung Sool DO
Author Information
1. School of Life Sciences, Handong Global University, Pohang, Gyungbuk, Korea. msdo@handong.edu
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
3T3-L1 adipocytes;
B-cell-activating factor;
coculture;
diet-induced obesity;
inflammation
- MeSH:
3T3-L1 Cells;
Adipocytes/drug effects/*metabolism;
Adipokines/genetics/*metabolism;
Adiponectin/genetics/metabolism;
Animals;
B-Cell Activating Factor/*metabolism/pharmacology;
Chemokine CCL2/genetics/metabolism;
Coculture Techniques;
Gene Expression Regulation/drug effects;
Haptoglobins/genetics/metabolism;
Inflammation Mediators/metabolism;
Interleukin-6/genetics/metabolism;
Leptin/genetics/metabolism;
Macrophages/drug effects/*metabolism;
Mice;
Mice, Inbred C57BL;
Mice, Obese;
RNA, Messenger/genetics/metabolism
- From:Experimental & Molecular Medicine
2013;45(1):e4-
- CountryRepublic of Korea
- Language:English
-
Abstract:
3T3-L1 adipocytes express the B-cell-activating factor (BAFF) and three different BAFF receptors (BAFF-Rs). Furthermore, BAFF expression is regulated by inflammatory modulators, such as tumor necrosis factor-alpha and rosiglitazone. Here we investigated the function of BAFF in 3T3-L1 adipocytes and RAW 264.7 macrophages. We examined adipokine expression in 3T3-L1 adipocytes treated with 10 ng ml-1 BAFF. We also examined inflammatory molecule expression in RAW 264.7 macrophages treated with 10 or 100 ng ml-1 BAFF. We examined BAFF expression in the coculture of 3T3-L1 adipocytes and RAW 264.7 macrophages, as well as in white adipose tissue (WAT) of diet-induced obese (DIO) mice. We found that BAFF decreases leptin and adiponectin expression, but increases the expression of proinflammatory adipokines monocyte chemotactic protein-1, interleukin-6 (IL-6), cyclooxygenase-2 (COX-2) and haptoglobin. Coculturing the two cell types resulted in increased BAFF mRNA and protein expression, as well as modulation of BAFF-R mRNA expression in both cell types. These data indicate that BAFF might mediate adipocyte and macrophage interaction. When RAW 264.7 macrophages were treated with BAFF, BAFF-R expression was modulated as in coculture, and nitric oxide synthase and IL-6 expression increased. BAFF expression also increased in WAT of DIO mice. We propose that BAFF can regulate adipokine expression and possibly mediate adipocyte and macrophage interaction.
