Identification of Plasma Coagulation Factor XIII, Transglutaminase 3 and N epsilon-(gamma-glutamyl) lysine cross-Link in the Silicotic Nodule by Immunohistochemistry.
- Author:
You Mie KIM
1
;
Young Jin KIM
;
Soo Young LEE
Author Information
1. Department of Natural Sciences, College of Medicine, The Catholic University, Korea. lsyng@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Transglutaminases(TGases);
Plasma coagulation factor XIII(F-XIII);
Silicotic nodule;
N epsilon-(gamma-glutamyl) lysine cross-link
- MeSH:
Antibodies;
Blood Coagulation Factors*;
Crystallins;
Factor XIII*;
Fibrosis;
Immunohistochemistry*;
Lung;
Lysine*;
Plasma*;
Silicon Dioxide
- From:Korean Journal of Occupational and Environmental Medicine
2003;15(2):173-180
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: This study was performed to examine the immunohistochemical distribution of TGase 1, 2, 3, coagulation factor XIII and N epsilon-(gamma-glutamyl) lysine cross-link in the silicotic nodules formed after an intratracheal instillation of the silica. METHODS: The immunohistochemical examinations used antibodies against TGase 1, 2, 3, coagulation factor XIII and N epsilon-(gamma-glutamyl) lysine isopeptide in the silicotic nodules induced after an intratracheal instillation of 50 mg of size fractionated, crystalline silica. RESULTS: A high level of TGase 3 was related to the severity of fibrosis in silicotic nodules and extracellular coagulation factor XIII was detected around the nodules. Expressions of both membrane-bound TGase 1 and TGase 2 were barely detected in the nodules although high expressions were detected in the intact lung. Formation of N epsilon-(gamma-glutamyl) lysine cross-links was increased in severe fibrotic nodules. CONCLUSIONS: TGase 3 might contribute to the eventual stone-like fibrosis via formation of N epsilon-(gamma-glutamyl) lysine cross-links. Futhermore, coagulation factor XIII plays a role in the formation of a provisional matrix which results in fibrogenesis during silicotic nodule formation.
