Effects of Glutathione on Cisplatin-Induced Cytotoxicity In Human Cervical Cancer Cell Lines.
- Author:
Tae Gyu AHN
1
;
Sei Jun HAN
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Chosun University, Kwangju, Korea.
- Publication Type:Original Article
- Keywords:
Cisplatin;
Cervical carcinoma cell;
Glutathione;
OTC
- MeSH:
Buthionine Sulfoximine;
Carcinoma, Squamous Cell;
Cell Line*;
Cisplatin;
Culture Media;
Cysteine;
Glutathione*;
Humans*;
Ligases;
Uterine Cervical Neoplasms*
- From:Korean Journal of Obstetrics and Gynecology
2002;45(8):1289-1293
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: The purpose of this study is to determine the effects of glutathione on cisplatin-induced cytotoxicity of human cervical carcinoma cell lines (SiHa: squamous cell carcinoma cell, CaSki: epidermoid metastatic carcinoma cell). METHODS: Human cervical carcinoma cells (SiHa, CaSki) were incubated with culture media (RPMI1640) in the presence of cisplatin and/or buthionine sulfoximine (BSO), as a inhibitor of gamma-glutamyl- cysteine synthetase, and/or glutathione (GSH) and/or 2-oxo 4-thiazolidine carboxylic acid (OTC). The viable cells were examined by using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) and was determined by spectrophotometer at 570 nm. RESULTS: The incubation of cervical cells with cisplatin resulted in an decreasing cells viability by dose response. The MTT reduction rate were not different by BSO (5 mM) treatment in cervical cell lines. The viable cells were increased significantly by glutathione (5 mM) or OTC (5 mM) in cisplatin-treated cell lines. CONCLUSION: gamma-glutamylcysteine synthetase inhibitor had no effect on cisplatin toxicity. GSH and OTC had effect on cisplatin cytotoxicity. So, These result suggested that cervical cancer line cells were more susceptive to protective effects of glutathione and OTC than BSO on cisplatin induced-toxicity.
