Evaluation for the sensitivity of LISS in antiglobulin test.
- Author:
Jong Weon CHOI
;
Jin Tae SUH
;
Cho Won CHUNG
- Publication Type:Original Article
- MeSH:
Anemia, Hemolytic;
Coombs Test*;
Humans;
Isoantibodies;
Osmolar Concentration
- From:Korean Journal of Blood Transfusion
1995;6(1):21-28
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The association rate of antibody with antigen has been reported to be greatly increased by lowering ionic strength. Low-ionic strength salt solution(LISS) method has been used for the detection of various alloantibodies. To investigate the sensitivity of LISS in indirect antiglobulin test, a comparison study of LISS with saline and albumin methods was conducted. A total of 32 patients' samples requested for indirect antiglobulin test were evaluated. Out of 32 patients with clinical immune hemolytic anemia, 11(34.3%) were positive in 37 degrees C saline antiglobulin test, 18(56.2%) in albumin antiglobulin test, 23(71.8%) in LISS antiglobulin test respectively. These results were statistically analyzed using non parametric Page's test for ordered alternatives. LISS method is more sensitive than 37 degrees C saline method or albumin method significantly (p<0.01).
