Regulation of retinal angiogenesis by phospholipase C-β3 signaling pathway.

Author: Jung Min HA ¹ ; Seung Hoon BAEK ; Young Hwan KIM ; Seo Yeon JIN ; Hye Sun LEE ; Sun Ja KIM ; Hwa Kyoung SHIN ; Dong Hyung LEE ; Sang Heon SONG ; Chi Dae KIM ; Sun Sik BAE
Author Information

1. Department of Pharmacology, Gene and Cell Therapy Center for Vessel-Associated Disease, Medical Research Institute, Pusan National University School of Medicine, Yangsan, Republic of Korea. sunsik@pusan.ac.kr
Publication Type:Original Article
MeSH: Animals; Blood Vessels; Endothelial Cells; Hemorrhage; Human Umbilical Vein Endothelial Cells; Mice; Phospholipases*; Retinaldehyde*
From:Experimental & Molecular Medicine 2016;48(6):e240-
CountryRepublic of Korea
Language:English
Abstract: Angiogenesis has an essential role in many pathophysiologies. Here, we show that phospholipase C-β3 (PLC-β3) isoform regulates endothelial cell function and retinal angiogenesis. Silencing of PLC-β3 in human umbilical vein endothelial cells (HUVECs) significantly delayed proliferation, migration and capillary-like tube formation. In addition, mice lacking PLC-β3 showed impaired retinal angiogenesis with delayed endothelial proliferation, reduced endothelial cell activation, abnormal vessel formation and hemorrhage. Finally, tumor formation was significantly reduced in mice lacking PLC-β3 and showed irregular size and shape of blood vessels. These results suggest that regulation of endothelial function by PLC-β3 may contribute to angiogenesis.