Expression and Cellular Localization of Psx Gene in Rat Placenta.
10.11637/kjpa.2011.24.1.51
- Author:
Eun Hwa CHOI
1
;
Chaeyong JUNG
;
Kwang Il NAM
;
Seung Won LEE
;
Choon Sang BAE
;
Baik Yoon KIM
;
Sung Sik PARK
;
Kyu Youn AHN
Author Information
1. Department of Anatomy, Chonnam National University Medical School, Research Institute of Medical Science, Chonnam National University, Korea. kyahn@jnu.ac.kr
- Publication Type:Original Article
- Keywords:
Placenta specific homeobox gene;
Placenta;
Gene expression;
in situ hybridization histochemistry
- MeSH:
Animals;
Chimera;
Chorion;
Conserved Sequence;
Decidua;
Ear, Inner;
Ectoderm;
Embryonic Structures;
Female;
Gene Expression;
Genes, Homeobox;
Giant Cells;
In Situ Hybridization;
Morphogenesis;
Organogenesis;
Placenta;
Placentation;
Rats;
RNA, Messenger;
Trophoblasts
- From:Korean Journal of Physical Anthropology
2011;24(1):51-56
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Homeobox genes seem to play critical roles in regulating morphogenesis, patterning, organogenesis, and differentiation. They have the conserved sequence that codes the DNA-binding domain called homeodomain. The expression and cellular localization of rPsx mRNA in rat placenta during placental development were examined by in situ hybridization histochemistry at different embryonic stages (Embryonic days 7.5~16.5). rPsx mRNA was first detected in chorionic ectoderm of placenta at E 10.5. This transcript was localized in labyrinth trophoblast and trophoblast giant cells at E 11.5. Hybridization signals were observed in labyrinth trophoblast, spongiotrophoblast, and trophoblast giant cells at E 12.5, E 13.5, and E 14.5. At E 15.5, hybridization signal was detected in labyrinth trophoblast and spongiotrophoblast but not in trophoblast giant cells. Hybridization signal was only detected in labyrinth trophoblast at E 16.5. rPsx mRNA was not detected in decidua and any tissues of the embryo from E 7.5 to E 9.5 of gestations. From these results, a new rPsx homeobox gene is first expressed at E 10.5 and detected in chorionic ectoderm, labyrinth trophblast, spongiotrophoblast and trophoblast giant cells of the placenta. This gene may play a critical role in differentiation and development of trophoblast cells.
