The Effects of Phlomis umbrosa Turcz on Osteoclast Differentiation.
10.11637/kjpa.2013.26.3.115
- Author:
Jong Min BAEK
1
;
Ju Ha PARK
;
Jeong Hoon SONG
;
Seong Nam PARK
;
Woo Jin JEONG
;
Seo Young MOON
;
Chang Hoon LEE
;
Min Kyu CHOI
;
Jeong Joong KIM
;
Jaemin OH
;
Sung Jo JANG
Author Information
1. Department of Anatomy and Institute for Skeletal Disease, School of Medicine, Wonkwang University, Korea.
- Publication Type:Original Article
- Keywords:
Phlomis umbrosa Turcz;
Osteoclast;
Osteoporosis;
Bone resorption;
RANKL
- MeSH:
Acid Phosphatase;
Actins;
Arthritis, Rheumatoid;
Biological Agents;
Bone Diseases;
Bone Marrow;
Bone Resorption;
Cathepsin K;
Cell Adhesion;
Cytokines;
Isoenzymes;
Macrophage Colony-Stimulating Factor;
Macrophages;
NF-kappa B;
Osteoclasts;
Osteoporosis;
Phlomis;
RANK Ligand;
RNA, Messenger;
T-Lymphocytes
- From:Korean Journal of Physical Anthropology
2013;26(3):115-123
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Many bone-related diseases such as osteoporosis, rheumatoid arthritis are occurred by excessive bone resorbing activity of osteoclasts. Recently, many studies have been proceeded to find out the new therapeutic materials from natural products of plants. Phlomis umbrosa Turcz, one of the natural products of plants has been known to improve bone health. However, the precise effects and treatment mechanisms of Phlomis umbrosa Turcz about bone diseases has been unknown. So, we examined the effects of Phlomis umbrosa Turcz on expression of receptor activator of nuclear factor-kappaB ligand (RANKL)-induced osteoclast differentiation in bone marrow-derived macrophages (BMMs) and bone resorption. Also, we investigated the treatment mechanisms of Phlomis umbrosa Turcz relating to osteoclast differentiation. Here, we showed that Phlomis umbrosa Turcz significantly suppressed RANKL-induced osteoclast differentiation and bone resportion. Furthermore, Phlomis umbrosa Turcz suppressed the activation of NF-kappaB in bone marrow macrophage treated RANKL and M-CSF. The mRNA expression of c-Fos, nuclear factor of activated T-cells (NFAT)c1, osteoclast-associated receptor (OSCAR), tartrate-resistant acid phosphatase (TRAP) in BMMs was inhibited by Phlomis umbrosa Turcz. Integrin alphanu, beta3 relating to cell adhesion and dendritic cell-specific transmembrane protein (DC-STAMP) relating to the structure of filamentous actin (F-actin) ring and cathepsin K relating to bone resorbing activity are disrupted too. These results suggest that Phlomis umbrosa Turcz will be a good materials to treat bone diseases like osteoporosis.
