A PCR-RFLP method for the detection of activated H-ras oncogene with a point mutation at codon 12 and 61.
10.3349/ymj.1996.37.6.371
- Author:
Sung Joon HONG
1
;
Tack LEE
;
Young Sug PARK
;
Kyung Ok LEE
;
Byung Ha CHUNG
;
Soo Hyung LEE
Author Information
1. Department of Urology, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
H-ras gene;
bladder tomor;
point mutation;
PCR-RFLP
- MeSH:
Base Sequence;
Bladder Neoplasms/genetics;
Carcinoma, Transitional Cell/genetics;
Codon;
*Gene Expression Regulation;
*Genes, ras;
Human;
Molecular Sequence Data;
*Point Mutation;
*Polymerase Chain Reaction;
*Polymorphism, Restriction Fragment Length
- From:Yonsei Medical Journal
1996;37(6):371-379
- CountryRepublic of Korea
- Language:English
-
Abstract:
To investigate the incidence of the H-ras gene activation in bladder tumor and the feasibility of using urinary washout samples for screening, a series of 33 human bladder tumors and their preoperatively collected urinary washout samples were screened using a mutant specific PCR-RFLP (polymerase chain-restriction fragment length polymorphism) to detect a point mutation of the H-ras gene. Five tumors were found to harbor H-ras mutations where two tumors had a glycine to valine (G-->T) change in codon 12 and three tumors had a glutamine to lysine (C-->A) change in codon 61, respectively. Moreover, we could also detect the same point mutations of the H-ras gene in corresponding urine washout samples. The incidence of H-ras mutation in Korean bladder cancer was estimated at approximately 15.2%. In conclusion, a mutant specific PCR-RFLP method for the detection of H-ras gene mutation is useful for screening or postoperative follow-up of bladder tumor due to its simplicity and high specificity even in urinary samples.
