Effect of Interleukin-10 on Lipopolysaccahride/Interferon-gamma- Induced Chemokine Mig Gene Expression.
- Author:
Jin Hee CHOI
1
;
Jung Sook JIN
;
Ho Sun PARK
;
Sung Kwang KIM
;
Jai Youl LEE
;
Hee Sun KIM
Author Information
- Publication Type:Original Article
- Keywords: IL-10; Mig; LPS; IFN-gamma; gene expression
- MeSH: Animals; Gene Expression*; Interferon-gamma; Interleukin-10*; Macrophages; Macrophages, Peritoneal; Mice; RNA, Messenger
- From:Immune Network 2002;2(1):12-18
- CountryRepublic of Korea
- Language:Korean
- Abstract: Interferon-gamma (IFN-gamma) is well known as a potent inducer in monokine induced by IFN-gamma (Mig) mRNA expression. Although lipopolysaccharide (LPS) alone is weakly effective on Mig mRNA expression. the stimulation of LPS and IFN-gamma (LPS/IFN-gamma simultaneously has been shown to synergize to produce a high level of Mig mRNA in mouse peritoneal macrophages. In this study, interleukin-10 (IL-10) was found to suppress the LPS/IFN-gamma- induced Mig mRNA expression in cell type- and mouse strain-specific fashion, but IFN-gamma alone-induced Mig mRNA was unaffected by IL-10 under identical experimental conditions. The IL-10-mediated suppression of LPS/IFN-gamma-stimulated Mig mRNA expression was dependent on the concentration of IL-10, and was prevented when the agent was added 2 hours after LPS/IFN-gamma treatment. The suppressive action of IL-10 was dependent on a protein synthesis. However, IL-10 did not reduce the stability of LPS/IFN-gamma-induced Mig mRNA. These data may have important implications for a previously unrecognized role for IL-10 as a regulator of synergistic effect of LPS on the IFN-gamma-induced expression of the Mig gene in macrophages.
