Analysis of Cellular Changes Resulting from Forced Expression of Dickkopf-1 in Hepatocellular Carcinoma Cells.
- Author:
Mi Hee KWACK
1
;
Sun Young HWANG
;
In Seok JANG
;
Sang Uk IM
;
Jin Oh KIM
;
Moon Kyu KIM
;
Jung Chul KIM
;
Young Kwan SUNG
Author Information
1. Department of Immunology, School of Medicine, Kyungpook National University, Daegu, Korea. ysung@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Apoptosis;
Cell adhesion;
Cell line;
Dickkopf-1;
Hepatocellular carcinoma
- MeSH:
Apoptosis;
Biology;
Carcinoma, Hepatocellular*;
Cell Adhesion;
Cell Line;
Collagen Type I;
Fibronectins
- From:Cancer Research and Treatment
2007;39(1):30-36
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Recent studies have shown that Dickkopf-1 (DKK-1) is overexpressed in some tumors, including hepatocellular carcinoma. However, the role of increased DKK-1 in these tumors is not known. In this study, the DKK-1 expression in hepatocellular carcinoma (HCC) cell lines was evaluated and the effect of DKK-1 overexpression in HCC cell lines was studied. MATERIALS AND METHODS: The expression of DKK-1 in hepatocellular carcinoma cell lines was evaluated by RT-PCR. Stable cell lines that overexpressed DKK-1 were established. Cell growth, adhesion, migration and invasion assays were performed. RESULTS: RT-PCR analysis showed that 5 out of 8 HCC cell lines expressed DKK-1. The forced expression of DKK-1 suppressed the growth of cells and increased the population of cells in the sub-G1 phase. In addition, DKK- 1 reduced the cellular adhesion capacity to collagen type I and fibronectin, and it increased migratory capacity. However, overexpression of DKK-1 did not increase the invasion capacity of the HCC cell line. CONCLUSION: Collectively, our data suggest that overexpression of DKK-1 affects the biology of HCC cells.
