Oxidative Damage induced by UV in Cultured Retinal Pigment Epithelial Cells.
- Author:
Hyeong Gon YU
1
;
Kyung Seek CHOI
;
Jang Won HEO
;
Kwan Chang KIM
;
Young Joo KIM
;
Yesun CHUNG
;
Young Suk YU
;
Hum CHUNG
Author Information
1. Department of Ophthalmology, Seoul National University, College of Medicine, Seoul Artificial Eye center, Seoul National University Hospital, Clinical Research Institute, Korea. chungh@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Apoptosis;
Oxidative damage;
Retinal pigment epithelium;
UV
- MeSH:
Annexin A5;
Apoptosis;
Cell Death;
Epithelial Cells*;
Flow Cytometry;
Humans;
Macular Degeneration;
Oxygen;
Propidium;
Retinal Pigment Epithelium;
Retinaldehyde*;
Trypan Blue
- From:Journal of the Korean Ophthalmological Society
2003;44(12):2890-2895
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The death of retinal pigment epithelial(RPE) cells plays an important role in the development of age-related macular degeneration. We studied whether ultraviolet could induce apoptotic cell death and the increase of reactive oxygen intermediates (ROI) in the RPE cells. METHODS: Cultured human RPE cells were exposed to doses of between 0 and 80 mJ/cm2 UVB as an attached monolayer. The number of viable cells was assessed by trypan blue assay. The apoptosis was determined by flow cytometry using annexin V and propidium iodide (PI). Intracellular ROI was measured using DCFH-DA. RESULTS: UVB-exposed cells showed a dose-dependent cell death. The number of cells decreased in 20~30% at the dose of 40 mJ/cm2 UVB 24 hours after UVB exposure. The proportion of annexin V positive cells and intracellular ROI increased after UVB exposure. CONCLUSIONS: These results suggest that UV-induced oxidative damage may be one of the important mechanisms of RPE cell death.
