The dose of cyclophosphamide for treating paraquat-induced rat lung injury.
10.3904/kjim.2013.28.4.420
- Author:
Jae Sung CHOI
1
;
Sung Shick JOU
;
Mee Hye OH
;
Young Hee KIM
;
Min Ju PARK
;
Hyo Wook GIL
;
Ho Yeon SONG
;
Sae Yong HONG
Author Information
1. Department of Internal Medicine, Soonchunhyang University Cheonan Hospital, Cheonan, Korea. hwgil@schmc.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Paraquat;
Cyclophosphamide;
Reactive oxygen species;
X-ray microtomography
- MeSH:
Animals;
Catalase/metabolism;
Cyclophosphamide/*pharmacology;
Cytokines/metabolism;
Disease Models, Animal;
Dose-Response Relationship, Drug;
Immunosuppressive Agents/*pharmacology;
Inflammation Mediators/metabolism;
Lung/*drug effects/metabolism/pathology/radiography;
Lung Injury/chemically induced/diagnosis/*drug therapy/metabolism;
Male;
Oxidative Stress/drug effects;
*Paraquat;
Pulmonary Edema/chemically induced/diagnosis/*drug therapy/metabolism;
Rats;
Rats, Sprague-Dawley;
Severity of Illness Index;
Superoxide Dismutase/metabolism;
Transforming Growth Factor beta1/metabolism;
X-Ray Microtomography
- From:The Korean Journal of Internal Medicine
2013;28(4):420-427
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: Cyclophosphamide (CP) is a promising treatment for severe cases of paraquat (PQ) poisoning. We investigated the effective dose of CP for mitigating PQ-induced lung injury. METHODS: Adult male Sprague-Dawley rats were allocated into five groups: control, PQ (35 mg/kg, intraperitoneal injection), and PQ + CP (1.5, 15, or 30 mg/kg). The dimensions of lung lesions were determined using X-ray microtomography (micro-CT), and histological changes and cytokine levels were recorded. RESULTS: The micro-CT results showed that 15 mg/kg CP was more effective than 1.5 mg/kg CP for treating PQ-induced lung injury. At a dose of 1.5 mg/kg, CP alleviated the histological evidence of inflammation and altered superoxide dismutase activity. Using 15 mg/kg CP reduced the elevated catalase activity and serum transforming growth factor (TGF)-beta1 level. CONCLUSIONS: A CP dose of > 15 mg/kg is effective for reducing the severity of PQ-induced lung injury as determined by histological and micro-CT tissue examination, possibly by modulating antioxidant enzyme and TGF-beta1 levels.
