Soluble Interleukin-2 Receptor(sIL-2R) Levels in Patients Tuberculous Pleurisy VS Nontuberculous Pleurisy.
10.4046/trd.1994.41.2.135
- Author:
Hyun Oak LIM
;
Jong Yeol HAM
;
Dae Seok SHIM
;
Young Sil HWANG
- Publication Type:Original Article
- Keywords:
Tuberculous pleurisy;
Nontuberculous pleurisy;
Soluble interleukin-2 receptor
- MeSH:
Autoimmune Diseases;
Diagnosis, Differential;
Humans;
Immunity, Cellular;
Immunoenzyme Techniques;
Interleukin-2*;
Lymphocyte Activation;
Lymphoproliferative Disorders;
Organ Transplantation;
Pleural Effusion;
Pleural Effusion, Malignant;
Pleurisy*;
Sensitivity and Specificity;
T-Lymphocytes;
Transplants;
Tuberculosis;
Tuberculosis, Pleural*
- From:Tuberculosis and Respiratory Diseases
1994;41(2):135-143
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The cell mediated immunity has an important role in the pathogenesis of tuberculosis. sIL-2R has been known as a sensitive marker of T lymphocyte activation. Elevated serum levels of sIL-2R have been found in patients with lymphoproliferative disorders, organ transplantation, autoimmune diseases, and various granulomatous diseases. Elevated levels of sIL-2R have been also found in the seam and pleural fluid of the patients with tuberculosis. To evaluate the diagnostic value of sIL-2R in the differentiation of tuberculous pleurisy and. nontuberculous pleurisy. We measured the level of sIL-2R in the sera and pleural fluids of 12 patients with tuberculous pleurisy and 32 patients with nontuberculous pleurisy. METHOD: Samples of pleural fluid and serum were centrifuged at 2500 rpm for 10 min to remove cell pellets. Soluble R-2R was measured with a sandwitch enzyme immunoassay using the Cellfree r Interleukin-2 Receptor Test kit( T-cell science, Inc. Cambridge, MA). RESULTS: The results obtained were as follows: 1) The sIL-2R level in pleural fluid of the patients with tuberculous pleurisy was higher than that of patients with nontuberculous pleurisy(P<0.005). 2) When the sIL-2R level above 5,000 u/ml in pleural fluid was used as the cut-off value to diagnose tuberculous pleurisy, it had a sensitivity of 84.6% and a specificity of 90.9%. 3) The sIL-2R level in the sera of the patients with tuberculous pleurisy was higher than that of patients with bacterial pleural effusions and normal control group(P<0.05) and there was no difference of levels compared with malignant pleural effusions and transudative pleural effusions(P>0.05). 4) In patients with tuberculous pleurisy, the mean concentration of sIL-2R in pleural fluid was higher than that in serum(P<0.005). CONCLUSION: These findings suggest that the measurement of elevated levels of pleural fluid sIL-2R in tuberculous pleurisy may be useful in the differential diagnosis between patients with tuberculous pleurisy and nontuberculous pleurisy.
