Over-expression of extracellular superoxide dismutase in mouse synovial tissue attenuates the inflammatory arthritis.
- Author:
Dong Hoon YU
1
;
Jun Koo YI
;
Hyung Soo YUH
;
Seo jin PARK
;
Hei Jung KIM
;
Ki Beom BAE
;
Young Rae JI
;
Na Ri KIM
;
Si Jun PARK
;
Do Hyung KIM
;
Sung Hyun KIM
;
Myoung Ok KIM
;
Jeong Woong LEE
;
Zae Young RYOO
Author Information
1. School of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Korea. jaewoong64@hanmail.net
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
arthritis, experimental;
reactive oxygen species;
rheumatoid arthritis;
superoxide dismutase;
synovial membrane
- MeSH:
Animals;
Arthritis, Experimental/blood/*enzymology/metabolism;
*Arthritis, Rheumatoid/enzymology/pathology;
Fibroblasts/metabolism;
Gene Expression Regulation;
Inflammation/pathology;
Interleukin-1beta/blood/metabolism;
Joints/enzymology/pathology;
Matrix Metalloproteinases/blood/metabolism;
Mice;
Mice, Transgenic;
Reactive Oxygen Species/metabolism;
*Superoxide Dismutase/genetics/metabolism;
Synovial Fluid/*enzymology;
Synovial Membrane/pathology
- From:Experimental & Molecular Medicine
2012;44(9):529-535
- CountryRepublic of Korea
- Language:English
-
Abstract:
Oxidative stress such as reactive oxygen species (ROS) within the inflamed joint have been indicated as being involved as inflammatory mediators in the induction of arthritis. Correlations between extracellular-superoxide dismutase (EC-SOD) and inflammatory arthritis have been shown in several animal models of RA. However, there is a question whether the over-expression of EC-SOD on arthritic joint also could suppress the progression of disease or not. In the present study, the effect on the synovial tissue of experimental arthritis was investigated using EC-SOD over-expressing transgenic mice. The over-expression of EC-SOD in joint tissue was confirmed by RT-PCR and immunohistochemistry. The degree of the inflammation in EC-SOD transgenic mice was suppressed in the collagen-induced arthritis model. In a cytokine assay, the production of pro-inflammatory cytokines such as, IL-1beta, TNFalpha, and matrix metalloproteinases (MMPs) was decreased in fibroblast-like synoviocyte (FLS) but not in peripheral blood. Histological examination also showed repressed cartilage destruction and bone in EC-SOD transgenic mice. In conclusion, these data suggest that the over-expression of EC-SOD in FLS contributes to the activation of FLS and protection from joint destruction by depressing the production of the pro-inflammatory cytokines and MMPs. These results provide EC-SOD transgenic mice with a useful animal model for inflammatory arthritis research.
