Dissemination of CTX-M Type Extended-Spectrum beta-Lactamases and Emergence of CTX-M-12 in Escherichia coli.
- Author:
Chi Eun OH
1
;
Jong Sik HONG
;
Il Kwon BAE
;
Eun Hyang SONG
;
Seok Hoon JEONG
;
Kyung Won LEE
;
Dong Eun YONG
;
Jong Wook LEE
;
Wee Gyo LEE
;
Jung Oak KANG
;
Ji Young AHN
;
Seong Geun HONG
;
Jong Hee SHIN
;
Young UH
;
Yeon Jun PARK
;
Eui Chong KIM
;
Hyo Sun KWAK
;
Gun Jo WOO
Author Information
1. Department of Pediatrics, Kosin University College of Medicine, Busan, Korea.
- Publication Type:Original Article
- Keywords:
Escherichia coli;
Extended-spectrum beta-lactamase;
CTX-M-12;
CTX-M-14;
CTX-M-15
- MeSH:
Agar;
beta-Lactamases*;
Diffusion;
Escherichia coli*;
Genotype;
Korea;
Polymerase Chain Reaction;
Prevalence
- From:The Korean Journal of Laboratory Medicine
2005;25(4):252-258
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Clinical isolates of Escherichia coli were evaluated to determine the prevalence and genotypes of Ambler class A extended-spectrum beta -lactamases (ESBLs). METHODS: Clinical isolates of E. coli were collected from 12 hospitals from February through July, 2004. Antimicrobial susceptibility was tested by disk diffusion and agar dilution methods, and ESBLproduction was determined by double-disk synergy test. TEM, SHV, CTX-M, PER-1, VEB, IBC, GES, and TLA type ESBL genes were detected by PCR amplifications, and the PCR products were subjected to direct sequencing. RESULTS: The double-disk synergy test was positive in 90.9% (149 in 164) of the ceftazidime- or cefotaxime-resistant E. coli isolates. The most prevalent types of Ambler class A ESBLs in E. coliisolates were CTX-M-15 (n=53). CTX-M-14 (n=24), CTX-M-3 (n=9), CTX-M-9 (n=3), CTX-M-12 (n=3), SHV-2a (n=1), SHV-12 (n=5) and TEM-52 (n=3) were also found. CTX-M-12 ESBL had never been reported before in Korea. CONCLUSIONS: CTX-M type ESBL-producing E. coli isolates are spreading and CTX-M-12 is emerging in Korea.
