Molecular Characteristics of B Subgroups in Koreans.
- Author:
Dong Hee SEO
1
;
Sung Yeun KIM
;
Ji Yeon KIM
;
Sung Sup PARK
;
Jung Bin LEE
;
Kyou Sup HAN
Author Information
1. Department of Blood Service Headquarters and Central Blood Laboratory Center, Korea.
- Publication Type:Original Article
- Keywords:
B subgroup;
ABO gene;
Allele;
Polymorphism;
Koreans
- MeSH:
Alleles;
Asian Continental Ancestry Group;
Blood Donors;
Exons;
Genetic Structures;
Humans;
Phenotype;
Polymerase Chain Reaction;
Red Cross
- From:The Korean Journal of Laboratory Medicine
2005;25(4):280-284
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: An accurate ABO blood type is essential for transfusion therapy. Genetic structures of ABO blood group and subgroup have been investigated and so far about 100 ABO alleles have been reported worldwide. This study was performed to investigate the molecular characteristics of B subgroups in the Korean population. METHODS: A total of 19 samples of B subgroups were collected from patients (n=11) and from blood donors (n=8) of Korean Red Cross blood centers; these samples had been typed serologically for the ABO blood group. Allele-specific polymerase chain reaction (PCR), direct sequencing of exon 6 and 7, and allele separation were performed for ABO gene analysis. RESULTS: The ABO PCR-RFLP genotyping results of 18 samples among the provisional 19 B subgroups were identical regardless of their phenotypes. Two new B alleles showing 255C>T base change and 547G>A base change were observed in B3 and A1B3 subgroups. CONCLUSIONS: Serologically unidentified B subgroups were unequivocally identified through molecular analyses of the ABO gene. And new ABO alleles observed only in the Korean B subgroups were recognized.
