Functional improvement of porcine neonatal pancreatic cell clusters via conformal encapsulation using an air-driven encapsulator.
10.3858/emm.2012.44.1.001
- Author:
Sol Ji PARK
1
;
Soojeong SHIN
;
Ok Jae KOO
;
Joon Ho MOON
;
Goo JANG
;
Curie AHN
;
Byeong Chun LEE
;
Young Je YOO
Author Information
1. Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Korea. bclee@snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
alginic acid;
diabetes mellitus type 1;
insulin-secreting cells;
islets of Langerhans transplantation;
tissue therapy
- MeSH:
Alginates/chemistry/metabolism;
Animals;
Animals, Newborn;
Capsules/chemistry;
Cell Survival;
Diabetes Mellitus/pathology/*therapy;
Disease Models, Animal;
Glucuronic Acid/chemistry/metabolism;
Graft Rejection/etiology/*prevention & control;
Hexuronic Acids/chemistry/metabolism;
Humans;
Insulin/secretion;
Islets of Langerhans/*metabolism/pathology;
Islets of Langerhans Transplantation/*methods;
Postoperative Complications/etiology/*prevention & control;
*Swine
- From:Experimental & Molecular Medicine
2012;44(1):20-25
- CountryRepublic of Korea
- Language:English
-
Abstract:
Transplantation of islet cells into diabetic patients is a promising therapy, provided that the islet cells are able to evade host immune rejection. With improved islet viability, this strategy may effectively reverse diabetes. We applied 2% calcium alginate to generate small and large capsules to encapsulate porcine neonatal pancreatic cell clusters (NPCCs) using an air-driven encapsulator. After encapsulation, the viability was assessed at 1, 4, 7, 14 and 28 days and secretion of functional insulin in response to glucose stimulation were tested at days 14 and 28. Selective permeability of the small alginate capsules was confirmed using various sizes of isothiocyanate-labeled dextran (FITC-dextran). Encapsulation of NPCCs was performed without islet protrusion in the small and large capsules. The viability of NPCCs in all experimental groups was greater than 90% at day 1 and then gradually decreased after day 7. The NPCCs encapsulated in large capsules showed significantly lower viability (79.50 +/- 2.88%) than that of naive NPCCs and NPCCs in small capsule (86.83 +/- 2.32%, 87.67 +/- 2.07%, respectively) at day 7. The viability of naive NPCCs decreased rapidly at day 14 (75.67 +/- 1.75%), whereas the NPCCs encapsulated in small capsules maintained (82.0 +/- 2.19%). After 14 and 28 days NPCCs' function in small capsules (2.67 +/- 0.09 and 2.13 +/- 0.09) was conserved better compared to that of naive NPCCs (2.04 +/- 0.25 and 1.53 +/- 0.32, respectively) and NPCCs in large capsules (2.04 +/- 0.34 and 1.13 +/- 0.10, respectively), as assessed by a stimulation index. The small capsules also demonstrated selective permeability. With this encapsulation technique, small capsules improved the viability and insulin secretion of NPCCs without islet protrusion.
