Evaluation of immunocontraceptive vaccine composed of gonadotropin-releasing hormone conjugated with granulocyte-macrophage colony-stimulating factor in male rats.
10.14405/kjvr.2017.57.3.155
- Author:
Byung Joo PARK
1
;
Yong Hyun KIM
;
Hee Seop AHN
;
Sang Hoon HAN
;
Hyeon Jeong GO
;
Joong Bok LEE
;
Seung Yong PARK
;
Chang Seon SONG
;
Sang Won LEE
;
In Soo CHOI
Author Information
1. Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea. ischoi@konkuk.ac.kr
- Publication Type:Original Article
- Keywords:
immunologic contraception;
gonadotropin-releasing hormone;
granulocyte-macrophage colony-stimulating factor;
rats;
vaccines
- MeSH:
Animals;
Antibodies;
Atrophy;
Castration;
Contraception, Immunologic;
Gonadotropin-Releasing Hormone*;
Gonads;
Granulocyte-Macrophage Colony-Stimulating Factor*;
Humans;
Male*;
Rats*;
Vaccines
- From:Korean Journal of Veterinary Research
2017;57(3):155-158
- CountryRepublic of Korea
- Language:English
-
Abstract:
Surgical castration performed to reduce male-associated problems results in pain and microbial infections in male animals. Therefore, immunocontraception, which is mediated by the animal's own antibodies against reproductive hormones, has been recommended as an alternative to surgical castration when considering the animal's welfare. In this study, a new immunocontraceptive vaccine composed of six tandem copies of gonadotropin-releasing hormone (GnRH) fused to rat granulocyte-macrophage colony-stimulating factor (GM-CSF) was developed, and its efficacy was evaluated in male rats. Three different doses (10, 50, and 100 µg) of recombinant GM-CSF–GnRH protein were injected three times at intervals of two weeks into male rats. The rats vaccinated with three doses of GM-CSF–GnRH produced a significantly higher level of antibodies against GnRH than that in the negative control rats. Severe atrophy of gonads was observed in rats vaccinated with three doses of GM-CSF–GnRH but not in the negative control rats. The results reveal that the new GnRH vaccine conjugated with rat GM-CSF induces efficient immunocontraception in male rats. This formulation of the immunocontraceptive vaccine would be applicable to both domestic and pet male animals.
