Isolation and identification of Moraxella cuniculi from a rabbit with keratoconjunctivitis.
10.14405/kjvr.2017.57.3.201
- Author:
Dong Kun YANG
1
;
Ha Hyun KIM
;
Jae Young YOO
;
Suk Kyung LIM
;
Soon Seek YOON
;
In Soo CHO
Author Information
1. Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs, Gimcheon 39660, Korea. yangdk@korea.kr
- Publication Type:Brief Communication
- Keywords:
Moraxella cuniculi;
identification;
isolation;
rabbits
- MeSH:
Agar;
Anti-Bacterial Agents;
Genes, rRNA;
Germany;
Keratoconjunctivitis*;
Microscopy, Electron, Scanning;
Moraxella*;
Rabbits;
Sequence Analysis;
Sheep;
Spectinomycin
- From:Korean Journal of Veterinary Research
2017;57(3):201-204
- CountryRepublic of Korea
- Language:English
-
Abstract:
A Gram-negative, catalase- and oxidase-positive, coccus-shaped bacterium was isolated from a rabbit with keratoconjunctivitis. Colonies of the isolate were round, smooth, and exhibited hemolytic activity on 5% sheep blood agar. Scanning electron microscopy revealed 0.4 to 0.5 µm diameter oval cocci. Partial 16S rRNA gene (1446 bp) sequence analysis demonstrated the isolate had significant homology with the Moraxella cuniculi CCUG2154 strain isolated from a rabbit in Germany in 1973. Our isolate was designated as APQAB1701. Antibiotic susceptibility tests demonstrated that APQAB1701 was sensitive to 24 antibiotics; 3 of the antibiotics (nalidixic acid, spectinomycin, and colistin) had minimal inhibitory concentrations ≥ 32 µg/mL against the isolate.
