Isolation of Phenolate Type Siderophore from Pseudomonas sp. PY002.
- Author:
Yeal PARK
;
Ho Sang KIM
;
Sun A CHOI
;
Kang RYU
;
Ji Young CHOI
- Publication Type:Original Article
- MeSH:
Absorption;
Hydrogen-Ion Concentration;
Magnetic Resonance Spectroscopy;
Phenol*;
Pseudomonas*;
Spectrum Analysis
- From:Journal of the Korean Society for Microbiology
1997;32(1):39-46
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Phenolate type siderophore was produced in Pseudomonas sp. PY002 (P. sp. PY002) which cultured in M9 minimal medium supplemented with 0 to 500 uM of dipyridyl. Pyochelin, a kind of siderophore, was detected as a single broad absorption band (280 nm) at pH 12.0, which is a characteristic of phenolate type siderophore. The 280 nm absorption spectrum of pyochelin was changed to 310 nm at pH 1.5. The pyochelin produced was a structurally unique phenolate siderophore, designated 2-[2-(o-hydroxyphenyl)-2-thiazolin-4-yl]-3-methyl-4-thiazolidine car- boxylic acid on the analysis of infrared radiation and 'H and ""C nuclear magnetic resonance spectroscopy. Additionally, purified pyochelin increased the cell growth rate, like as growth fac- tor. All these results suggest that phenolate type siderophore play an important role in cell growth of P. sp. PY002.
