Detection of M. hominis and U. urealyticum using Polymerase Chain Reaction in Clinical Samples.
- Author:
Eun Ha KIM
;
Mi Ja LEE
;
Jee Ae IM
;
Young Suk PARK
- Publication Type:Original Article
- MeSH:
Abortion, Spontaneous;
Cervix Uteri;
Chorioamnionitis;
Culture Media;
DNA;
Female;
Humans;
Infant;
Infant, Low Birth Weight;
Infant, Newborn;
Mothers;
Mycoplasma;
Mycoplasma hominis;
Polymerase Chain Reaction*;
Pregnancy;
Ureaplasma urealyticum;
Urethritis;
Vagina
- From:Journal of the Korean Society for Microbiology
1997;32(1):47-52
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Both Mycoplasma hominis (M. hominis) and Ureaplasma urealyticum (U. urealyticum) are important genital mycoplasmas which are found in the cervix, vagina and the urogenital tracts of females. It has been associated with various disease states including non-gonococcal urethritis, spontaneous abortion, low birth weight and chorioamnionitis. Moreover they can be transmitted up to 40% of infants born to infected mothers. The culture methods which is commonly used for the determination of above mycoplasmas requires special culture media and can take up to 7 days. Recently the polymerase chain reaction (PCR) has been successfully applied for the detection of pathogenic microorganisms. In this study, M. hominis and U. urealyticum were determined using PCR in 70 vaginal swabs and 48 urine samples. In a total of seventy vaginal swabs, thirteen (18.5%) and twenty four (34.3%) samples were positive for M. hominis and U. urealyticum, respectively. In forty eight urine samples, M. hominis and U. urealyticum were found in 3 cases (6.3%), respectively. Among vaginal swab and urine samples, one case was found to be coinfectious state. Amplified DNA bands were more frequently found in vaginal swabs than urine samples. This study shows that PCR is a relatively simple, fast and practical tool for the detection of M. hominis and U. urealyticum in a clinical materials instead of culture method.
