Enhancement of Human Monocyte Activities against Aspergillus fumigatus by Interleukin-15.
- Author:
Jeong Ho CHO
1
;
Kyung Hee CHANG
;
Hyo Youl KIM
;
Young Goo SONG
;
Chang O KIM
;
Yoon Soo PARK
;
Sung Kwan HONG
;
Aejung HUH
;
Joon Sup YEON
;
June Myung KIM
Author Information
1. Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea. jmkim@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
IL-15;
Aspergillus fumigatus;
Monocyte
- MeSH:
Adult;
Aspergillosis;
Aspergillus fumigatus*;
Aspergillus*;
Candida albicans;
Drug Therapy;
Homicide;
Humans*;
Hyphae;
Immunocompromised Host;
Interleukin-15*;
Monocytes*;
Mortality;
Spores, Fungal;
Superoxides;
Tetradecanoylphorbol Acetate
- From:Korean Journal of Medical Mycology
2001;6(2):64-69
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Despite recent advances in antifungal chemotherapy, invasive aspergillosis remains an important cause of morbidity and mortality in immunocompromised patients. Interleukin-15 (IL-15) is a cytokine that is known to enhance antifungal activities of monocytes against Candida albicans. OBJECTIVE: The purpose of this study is to investigate the potentials of IL-15 to enhance antifungal activities of monocytes against Aspergillus fumigatus. METHODS: Peripheral blood monocytes from healthy adults were incubated with 0, 1, 10, 100 ng/ml of IL-15 for 1, 2, and 4 days. Then, the ability of IL-15 to elicit the production of superoxide anion, the damage of hyphae by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and the killing ability of Aspergillus fumigatus conidia was investigated. RESULTS: Incubation of peripheral blood monocytes with 100 ng/ml of IL-15 enhanced hyphal damage after 2 days (p<0.05), conidicidal activity from the first day (p<0.05), and increased the production of superoxide anion (O2- ) in response to phorbol myristate acetate. CONCLUSION: Our results indicate that IL-15 augments the microbicidal activity of human monocytes against Aspergillus fumigatus.
