T Lymphocyte Subsets and Cytokines in Rats Transplanted with Adipose-Derived Mesenchymal Stem Cells and Acellular Nerve for Repairing the Nerve Defects.
10.3340/jkns.2015.58.2.101
- Author:
Liang Fu JIANG
1
;
Ou CHEN
;
Ting Gang CHU
;
Jian DING
;
Qing YU
Author Information
1. Department of Hand & Plastic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Zhejiang, China. LiangfuJiangcn@163.com
- Publication Type:Original Article
- Keywords:
Neural transplantation;
Immunity;
T cell subsets;
Cytokines
- MeSH:
Allografts;
Animals;
Autografts;
Cytokines*;
Enzyme-Linked Immunosorbent Assay;
Flow Cytometry;
Interferon-gamma;
Interleukin-2;
Mesenchymal Stromal Cells*;
Orbit;
Rats*;
Rats, Sprague-Dawley;
Rats, Wistar;
Sciatic Nerve;
T-Lymphocyte Subsets*;
Transplants;
Tumor Necrosis Factor-alpha
- From:Journal of Korean Neurosurgical Society
2015;58(2):101-106
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: The aim of this study was to explore the immunity in rats transplanted with adipose-derived mesenchymal stem cells (ADSCs) and acellular nerve (ACN) for repairing sciatic nerve defects. METHODS: ADSCs were isolated from the adipose tissues of Wistar rats. Sprague-Dawley rats were used to establish a sciatic nerve defect model and then divided into four groups, according to the following methods : Group A, allogenic nerve graft; Group B, allograft with ACN; Group C, allograft ADSCs+ACN, and Group D, nerve autograft. RESULTS: At the day before transplantation and 3, 7, 14, and 28 days after transplantation, orbital venous blood of the Sprague-Dawley rats in each group was collected to detect the proportion of CD3+, CD4+, and CD8+ subsets using flow cytometry and to determine the serum concentration of interleukin-2 (IL-2), tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) using enzyme-linked immunosorbent assay (ELISA). At each postoperative time point, the proportion of CD3+, CD4+, and CD8+ subsets and the serum concentration of IL-2, TNF-alpha, and IFN-gamma in group C were all near to those in group B and group D, in which no statistically significant difference was observed. As compared with group A, the proportion of CD3+, CD4+, and CD8+ subsets and the serum concentration of IL-2, TNF-alpha, and IFN-gamma were significantly reduced in group C (p<0.05). CONCLUSION: The artificial nerve established with ADSCs and ACN has no obvious allograft rejection for repairing rat nerve defects.
