Comparison of Serum Proteome Maps of Children with Kawasaki Disease.
- Author:
Seoung Yon BAEK
1
;
Young Mock LEE
;
Kwang Hoon LEE
;
Dong Soo KIM
Author Information
1. Department of Pediatrics, College of Medicine, Yonsei University, Seoul, Korea. dskim6634@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Kawasaki disease;
Proteomics;
Two-dimensional electrophoresis;
Proteome map
- MeSH:
Child*;
Computational Biology;
Electrophoresis;
Heart Diseases;
Humans;
Isoelectric Focusing;
Mucocutaneous Lymph Node Syndrome*;
Proteome*;
Proteomics;
Silver;
Spectrometry, Mass, Electrospray Ionization;
Systemic Vasculitis
- From:Korean Journal of Pediatrics
2004;47(1):81-89
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Kawasaki disease is a systemic vasculitis observed in children under five years of age and the leading cause of pediatric acquired heart disease, but its pathophysiology is still not completely understood. With proteomics, the study of quality and quantity of proteins, having been developed dramatically, we tried to find a way to evaluate the etiology and pathogenesis of Kawasaki disease by analyzing the serum proteome maps. METHODS: We studied 10 patients admitted to the Pediatric Department of Yonsei Severance Hospital from January 2000 to July 2001. Half were diagnosed as typical Kawasaki disease and the rest as upper respiratory infection. Sera collected from the patients were frozen, melted and rehydrated for isoelectric focusing method using polyacrylamide gel and two-dimensional electrophoresis. The gel were stained by the silver method and scanned with GS-800 Calibrated Imaging Densitometer. PDQuest was used to quantify protein and draw proteome maps. RESULTS: 15 protein spots of molecular weight(kDa)/isoelectric point 76.1/3.80, 75.0/4.70, 74.4/4.70, 66.3/5.77, 91.8/5.77, 125.5/5.84, 92.4/5.85, 83.8/6.09, 77.0/6.26, 76.8/6.39, 103.4/6.49, 126.5/6.40, 122.9/6.74, 35.3/6.18, 28.5/6.68 were significantly decreased in children with Kawasaki disease. CONCLUSION: Further evaluations using methods such as electrospray ionization mass spectrometry (ESI-MS), matrix-assisted laser desorption-ionization mass spectrometry(MALDI-MS) or SWISS-PROT(Swiss Institute of Bioinformatics, Geneva, Switzerland) would have to be performed to define and to understand the functions of those protein spots decreased in patients with Kawasaki disease.
