Canine model of ischemic stroke with permanent middle cerebral artery occlusion: clinical and histopathological findings.
- Author:
Byeong Teck KANG
1
;
Jong Hwan LEE
;
Dong In JUNG
;
Chul PARK
;
Su Hyun GU
;
Hyo Won JEON
;
Dong Pyo JANG
;
Chae Young LIM
;
Fu Shi QUAN
;
Young Bo KIM
;
Zang Hee CHO
;
Eung Je WOO
;
Hee Myung PARK
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: dog, histopathology; ischemic stroke; MCAO; MRI
- MeSH: Animals; Behavior, Animal/physiology; Brain/metabolism/pathology; Cerebral Infarction/*etiology/*pathology; Cerebrospinal Fluid/chemistry/cytology; Disease Models, Animal; *Dogs; Infarction, Middle Cerebral Artery/*complications/*pathology; Magnetic Resonance Imaging; Male
- From:Journal of Veterinary Science 2007;8(4):369-376
- CountryRepublic of Korea
- Language:English
- Abstract: The aim of the present study was to assess the clinical and histopathological findings in a canine model of ischemic stroke. Cerebral ischemic stroke was induced by middle cerebral artery occlusion in four healthy beagle dogs using silicone plugs. They showed neurological signs of forebrain dysfunction such as reduced responsiveness, head turning, circling, postural reaction deficits, perceptual deficits, and hemianopsia. These signs gradually regressed within 4 weeks without therapy. On magnetic resonance imaging, T2 hyperintensity and T1 hypointensity were found in the cerebral cortex and basal ganglia. These lesions were well-defined and sharply demarcated from adjacent brain parenchyma with a homogenous appearance. No abnormalities of the cerebrospinal fluid were observed. At necropsy, atrophic and necrotic lesions were observed in the cerebral cortex. The cerebral cortex, basal ganglia, and thalamus were partially unstained with triphenyl-tetrazolium chloride. Histopathologically, typical features of infarction were identified in cortical and thalamic lesions. This study demonstrates that our canine model resembles the conditions of real stroke patients.
