Enhanced tyrosine hydroxylase expression in PC12 cells co-cultured with feline mesenchymal stem cells.
- Author:
Guang Zhen JIN
1
;
Xi Jun YIN
;
Xian Feng YU
;
Su Jin CHO
;
Hyo Sang LEE
;
Hyo Jong LEE
;
Il Keun KONG
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: co-culture; feline bone marrow; mesenchymal stem cell; rat PC12 cell; tyrosine hydroxylase
- MeSH: Animals; Antigens, Surface/metabolism; Blotting, Western; Cats/*physiology; Cell Culture Techniques; Cells, Cultured; *Gene Expression Regulation, Enzymologic; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)/metabolism; Immunohistochemistry; Mesenchymal Stem Cells/*cytology/metabolism; Microscopy, Phase-Contrast; PC12 Cells/cytology/*enzymology; Rats; Tyrosine 3-Monooxygenase/*metabolism
- From:Journal of Veterinary Science 2007;8(4):377-382
- CountryRepublic of Korea
- Language:English
- Abstract: Mesenchymal stem cells (MSCs) secrete a variety of neuroregulatory molecules, such as nerve growth factor, brain-derived neurotrophic factor, and glial cell-derived neurotrophic factor, which upregulate tyrosine hydroxylase (TH) gene expression in PC12 cells. Enhancing TH gene expression is a critical step for treatment of Parkinson's disease (PD). The objective of this study was to assess the effects of co-culturing PC12 cells with MSCs from feline bone marrow on TH protein expression. We divided the study into three groups: an MSC group, a PC12 cell group, and the combined MSC + PC12 cell group (the co-culture group). All cells were cultured in DMEM-HG medium supplemented with 10% fetal bovine serum for three days. Thereafter, the cells were examined using western blot analysis and immunocytochemistry. In western blots, the co-culture group demonstrated a stronger signal at 60 kDa than the PC12 cell group (p < 0.001). TH was not expressed in the MSC group, either in western blot or immunocytochemistry. Thus, the MSCs of feline bone marrow can up-regulate TH expression in PC12 cells. This implies a new role for MSCs in the neurodegenerative disease process.
