Expression of Translationally Controlled Tumor Protein (TCTP) Gene of Dirofilaria immitis Guided by Transcriptomic Screening.
- Author:
Yan FU
1
;
Jingchao LAN
;
Xuhang WU
;
Deying YANG
;
Zhihe ZHANG
;
Huaming NIE
;
Rong HOU
;
Runhui ZHANG
;
Wanpeng ZHENG
;
Yue XIE
;
Ning YAN
;
Zhi YANG
;
Chengdong WANG
;
Li LUO
;
Li LIU
;
Xiaobin GU
;
Shuxian WANG
;
Xuerong PENG
;
Guangyou YANG
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: Dirofilaria immitis; heartworm; dirofilariasis; translationally controlled tumor protein; transcriptome
- MeSH: Animal Structures/chemistry; Animals; Antibodies, Helminth/blood; Antigens, Helminth/chemistry/*genetics/immunology/*isolation & purification; Cloning, Molecular; Dirofilaria immitis/chemistry/*genetics/immunology; Disease Models, Animal; Dogs; Escherichia coli/genetics; Gene Expression; Molecular Sequence Data; Molecular Weight; Recombinant Fusion Proteins/chemistry/genetics/immunology/isolation & purification; Sequence Analysis, DNA; Tumor Markers, Biological/chemistry/*genetics/immunology/*isolation & purification
- From:The Korean Journal of Parasitology 2014;52(1):21-26
- CountryRepublic of Korea
- Language:English
- Abstract: Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose.
