Impact of Lysophosphatidylcholine on the Plasminogen Activator System in Cultured Vascular Smooth Muscle Cells.
10.3346/jkms.2012.27.7.803
- Author:
Byung Koo YOON
1
;
Young Hee KANG
;
Won Jong OH
;
Kyungwon PARK
;
Dong Yun LEE
;
Dooseok CHOI
;
Duk Kyung KIM
;
Youngjoo LEE
;
Mee Ra RHYU
Author Information
1. Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. bkyoon@skku.edu
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Lysophosphatidylcholines;
NF-kappa B;
Oxidative Stress;
Plasminogen Activator Inhibitor 1;
Protein-Tyrosine Kinase;
Muscle, Smooth, Vascular
- MeSH:
Animals;
Benz(a)Anthracenes/pharmacology;
Caffeic Acids/pharmacology;
Cells, Cultured;
Genistein/pharmacology;
Lipoproteins, LDL/metabolism;
Lysophosphatidylcholines/*pharmacology;
Muscle, Smooth, Vascular/cytology/*drug effects/metabolism;
NF-kappa B/antagonists & inhibitors/metabolism;
Oxidative Stress/drug effects;
Phenylethyl Alcohol/analogs & derivatives/pharmacology;
Plasminogen Activator Inhibitor 1/agonists/genetics/*metabolism;
Protein Kinase Inhibitors/pharmacology;
Protein-Tyrosine Kinases/antagonists & inhibitors/metabolism;
Rats;
Rats, Sprague-Dawley;
Reactive Oxygen Species/metabolism;
Signal Transduction/drug effects;
Tissue Plasminogen Activator/*metabolism;
Transcription, Genetic/drug effects;
Up-Regulation/drug effects;
Vitamin E/pharmacology
- From:Journal of Korean Medical Science
2012;27(7):803-810
- CountryRepublic of Korea
- Language:English
-
Abstract:
The balance between tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor type 1 (PAI-1) regulates fibrinolysis. PAI-1 expression increases in atherosclerotic arteries and vascular smooth muscle cells (VSMCs) are one of major constituents of atheroma. We investigated the impact of lysophosphatidylcholine (lysoPC), an active component of oxidized low-density lipoprotein, on the plasminogen activator system of the rat VSMCs. The lysoPC stimulated the protein and gene expressions of PAI-1 but did not affect the protein expression of t-PA. Fibrin overlay zymography revealed that lysoPC increased the activity of PAI-1 in the conditioned media, while concurrently decreasing that of free t-PA. Vitamin E inhibited the lysoPC-induced PAI-1 expression. Further, lysoPC increased the intracellular reactive oxygen species (ROS) formation. Caffeic acid phenethyl ester, an inhibitor of NF-kappaB, blocked this lysoPC effect. Indeed, lysoPC induced the NF-kappaB-mediated transcriptional activity as measured by luciferase reporter assay. In addition, genistein, an inhibitor of protein-tyrosine kinase (PTK), diminished the lysoPC effect, while 7,12-dimethylbenz[a]anthracene, a stimulator of PTK, stimulated PAI-1 production. In conclusion, lysoPC does not affect t-PA expression but induces PAI-1 expression in the VSMC by mediating NF-kappaB and the genistein-sensitive PTK signaling pathways via oxidative stress. Importantly, lysoPC stimulates the enzyme activity of PAI-1 and suppresses that of t-PA.
