Clinical application of preimplantation genetic diagnosis (PGD) using fluorescence in-situ hybridization to balanced reciprocal or Robertsonian translocation carriers in human IVF-ET program.
- Author:
Jin Hyun JUN
;
Gyun Jee SONG
;
Jeong Wook KIM
;
So Yeon PARK
;
Kye Hyun KIM
;
Bum Chae CHOI
;
Mi Kyoung KOONG
;
Inn Soo KANG
;
Chun Kyu LIM
;
Mi Hyun HAN
- Publication Type:Original Article
- Keywords:
Preimplantation genetic diagnosis (PGD);
Fluorescence in-situ hybridization (FISH);
Balanced reciprocal translocation;
Robertsonian translocation;
IVF-ET;
Robertsonian
- MeSH:
Blastomeres;
Embryonic Structures;
Family Characteristics;
Female;
Fluorescence*;
Humans*;
Karyotype;
Male;
Oocytes;
Polar Bodies;
Pregnancy;
Preimplantation Diagnosis*;
Prostaglandins D;
Uterus
- From:Korean Journal of Obstetrics and Gynecology
2000;43(7):1147-1153
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: This study was performed to evaluate the efficiency of preimplantation genetic diagnosis (PGD) using fluorescence in-situ hybridization (FISH) in Robertsonian or balanced reciprocal translocation carriers in human IVF-ET programm. METHOD: FISH was carried out in 25 cycles of 15 couples. Two-color FISH analysis was performed on 54 polar bodies in 3 cycles and 234 blastomeres in 22 cycles. After FISH analysis, the embryos with normal FISH signals were transferred into mother's uterus. RESULTS: In FISH analysis of polar bodies, 18 nuclei of polar bodies were normal and 12 embryos were transferred in 3 cycles. FISH efficiency per oocyte was 95.0% in cases using polar bodies. In FISH analysis of blastomeres, 49 embryos were normal and transferred in 21 cycles. FISH efficiency per embryo was 92.7% using blastomeres. At present, three pregnancies were achieved. A girl and a boy were delivered. Both of them were translocation carriers. The other conceptus showed normal karyotype. CONCLUSIONS: According to this study, PGD using FISH can be successfully applied for the patients with translocations of chromosomes.
