Identification of Outer Membrane Vesicles Derived from Orientia tsutsugamushi.
10.3346/jkms.2015.30.7.866
- Author:
Sun Myoung LEE
1
;
Hea Yoon KWON
;
Jae Hyong IM
;
Ji Hyeon BAEK
;
Jae Seung KANG
;
Jin Soo LEE
Author Information
1. Translation Research Center, Inha University School of Medicine, Incheon, Korea.
- Publication Type:Original Article
- Keywords:
Orientia tsutsugamushi;
Scrub Typhus;
Outer Membrane Vesicles;
Antigens
- MeSH:
Antibodies, Monoclonal/*immunology;
Antigens, Bacterial/*immunology;
Antigens, Surface/*immunology;
Cell Line;
Cell Membrane/immunology;
Humans;
Microscopy, Electron;
Orientia tsutsugamushi/*immunology/metabolism;
Scrub Typhus/diagnosis/microbiology;
Secretory Vesicles/*immunology
- From:Journal of Korean Medical Science
2015;30(7):866-870
- CountryRepublic of Korea
- Language:English
-
Abstract:
Orientia tsutsugamushi, a causative pathogen of Scrub typhus, is a gram-negative intracellular bacterium. Outer membrane vesicles (OMVs) are produced from the membrane of bacteria and play many roles related to the survival of the pathogen. However, there have been no reports confirming whether O. tsutsugamushi indeed produce OMVs. O. tsutsugamushi boryong was cultured in ECV-304 cells for the purification of OMVs. Western blot analysis and immunoenrichment using anti-O. tsutsugamushi monoclonal antibody and electron microscopy were employed for identification and characterization of OMVs. We confirm the presence of OMVs derived from O. tsutsugamushi, and also found that those OMVs contain a major surface antigen of 56-kDa protein and variant immunogenic antigens.
