Effect of Antioxidants on FeSO4 Toxicity in Cultured Myocardial Cells.
10.11637/kjpa.1997.10.2.161
- Author:
Ock Kyu PARK
1
;
Jae Min OH
;
Min Kyu CHOI
;
Seung Taeck PARK
;
Yeun Tai CHUNG
Author Information
1. Department of Internal Medicine, School of Medicine, Wonkwang University, Iksan, Korea.
- Publication Type:Original Article
- Keywords:
FeSO4;
Antioxidant;
Cardiotoxicity;
Cultured myocardial cell
- MeSH:
Animals;
Antioxidants*;
Ascorbic Acid;
Cell Survival;
Cells, Cultured;
Glutathione;
Rats
- From:Korean Journal of Physical Anthropology
1997;10(2):161-168
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In order to elucidate the cardiotoxicity of FeSO4 in cultured myocardial cells derived from neonatal rat, cardiotoxicity was measured by MTT assay when cultured cells were treated with various concentrations of FeSO4. In addition, the cardioprotective effect of antioxidants, glutathione and ascorbic acid was evaluated by MTT assay in these cultrures. Cell viability was remakably decreased in a dose -dependent manner after exposure of cultured rat myocardial cells to 20 microM FeSO4 for 48 hours. In the cardioprotective effect of antioxidants on FeSO4 -induced toxicity, glutathione blocked the cardiotoxicity induced by FeSO4, while ascorbic acid was not effective in blocking FeSO4 -induced cardiotoxicity in these cultures. These results suggest that FeSO4 is toxic in cultured myocardial cells from neonatal rat and selective antioxidants such as glutathione are effective in blocking the cardiotoxicity induced by FeSO4.
