Transplantation of a Scaffold-Free Cartilage Tissue Analogue for the Treatment of Physeal Cartilage Injury of the Proximal Tibia in Rabbits.
10.3349/ymj.2016.57.2.441
- Author:
Sang Uk LEE
1
;
Jae Young LEE
;
Sun Young JOO
;
Yong Suk LEE
;
Changhoon JEONG
Author Information
1. Department of Orthopaedic Surgery, Incheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Incheon, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Cartilage tissue analogue;
chondrocyte;
growth plate injury;
angular deformity;
bone bridge;
surgical treatment
- MeSH:
Animals;
*Bone Transplantation;
Cartilage/anatomy & histology;
Cell Culture Techniques;
Cells, Cultured;
Chondrocytes/*cytology/transplantation;
Growth Plate/anatomy & histology/*surgery;
*Mesenchymal Stem Cell Transplantation;
Rabbits;
Tibia/*surgery;
Tissue Engineering;
Transplantation, Autologous/methods;
Transplantation, Homologous
- From:Yonsei Medical Journal
2016;57(2):441-448
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The purpose of this study was to investigate the effects of transplantation of an in vitro-generated, scaffold-free, tissue-engineered cartilage tissue analogue (CTA) using a suspension chondrocyte culture in a rabbit growth-arrest model. MATERIALS AND METHODS: We harvested cartilage cells from the articular cartilage of the joints of white rabbits and made a CTA using a suspension culture of 2x107 cells/mL. An animal growth plate defect model was made on the medial side of the proximal tibial growth plate of both tibias of 6-week-old New Zealand white rabbits (n=10). The allogenic CTA was then transplanted onto the right proximal tibial defect. As a control, no implantation was performed on the left-side defect. Plain radiographs and the medial proximal tibial angle were obtained at 1-week intervals for evaluation of bone bridge formation and the degree of angular deformity until postoperative week 6. We performed a histological evaluation using hematoxylin-eosin and Alcian blue staining at postoperative weeks 4 and 6. RESULTS: Radiologic study revealed a median medial proximal tibial angle of 59.0degrees in the control group and 80.0degrees in the CTA group at 6 weeks. In the control group, statistically significant angular deformities were seen 3 weeks after transplantation (p<0.05). On histological examination, the transplanted CTA was maintained in the CTA group at 4 and 6 weeks postoperative. Bone bridge formation was observed in the control group. CONCLUSION: In this study, CTA transplantation minimized deformity in the rabbit growth plate injury model, probably via the attenuation of bone bridge formation.
