A Study on the Catecholamine under the Room Temperature and 5 degrees C Refrigerator Environment in Rat.
- Author:
Seyng Eui HONG
;
Tai Young YOON
;
Hyung Suk KIM
- Publication Type:Original Article
- MeSH:
Ammonium Compounds;
Animals;
Catecholamines;
Chromatography, High Pressure Liquid;
Dopamine;
Fluorescence;
Rats*
- From:Korean Journal of Preventive Medicine
1987;20(2):215-220
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Under the extreme change of the environment, animals react physiologically to adapt to the stress and secrete catecholamines. Cold exposure is a kind of the environmental stress. Author tried to determine the amount of catecholamines in rat urine as a parameter of physiological response to cold stress. Urinary catecholamine was measured by using HPLC with fluorescence detector, coation exchange column prepacked with Bio-Rex 70 and ammonium pentaborate as catecholamine eluent. The amount of dopamine in normal state rat urine was 42.0 ng, but under the low temperature of 5 degrees C, the dopamine amount was increased to 221.25 ng/5 ml. Above findings are suggesting that catecholamine secretion, especially dopamine, increase in the stressful condition such as cold exposure.
